The Effect of ۶ Weeks of High-Intensity Interval Training on Expression of FTO in Visceral Fat and Insulin Levels in Diabetic Obese Male Rats

Background and Aim: Among the genes associated with obesity and diabetes, the FTO gene is known as the most influential gene. Exercise and physical activity are among the factors that can affect FTO gene expression. The aim of this study was to investigate the effect of ۶ weeks of high-intensity intermittent training (HIIT) on visceral fat FTO gene expression and plasma insulin levels in obese male diabetic rats. Methods: For this purpose, ۱۲ Wistar rats with a mean weight of ۲۲۰ ± ۲۰ grams were randomly divided into two groups: control (n = ۶) and exercise (n = ۶). Diabetes was induced by a high-fat diet and intraperitoneal injection of streptozotocin. HIIT exercise by running on a treadmill, performed five sessions per week for ۶ weeks, with ۸-۱۰ repetitions of ۴۰ seconds per session. Forty-eight hours after the last training session, blood sampling from the heart and visceral fat biopsy of rats were performed, and gene expression was analyzed by Real-Time PCR. To determine the significance of the differences between the variables in the two groups, the t-test and ANCOVA were used. Results: The results showed that the expression of the FTO gene in visceral fat of the exercise group was significantly reduced compared to the control group (P = ۰.۰۳). Plasma insulin, plasma glucose, and body weight were significantly decreased in the exercise group compared to the control group, but the insulin sensitivity index was significantly increased (P = ۰.۰۰۱). Conclusion: Based on the findings of the present study, it seems that six weeks of HIIT can help improve diabetes and obesity by reducing FTO gene expression and increasing insulin sensitivity.

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Multiple Sclerosis ، Rehabilitation ، Neuroplasticity ، Fatigue Management ، Gait and Balance Therapy