Inspect the Effects of Some Insects and a Centipede on the Control to the AGS/Gastric Cancer Cell

Background: Insects and arthropods, along with their products, have been used as medicinal sources since ancient time that is called entomotherapy. Cancer is one of the major problems in medical science, for which a drug has not yet been discovered to control it quickly and accurately. Arthropods have been medicinal sources since ancient times and they especially Insects have a high potential for insect therapy because of the biodiversity. This research investigates the effect of insect body extracts and arthropods in inhibiting the growth of cancer cells or tumours, specifically on the gastric cancer cell line, AGS. Methods: The basic and applied study was conducted in Isfahan from ۲۰۱۸ to ۲۰۲۳. In this research, six genuses of insects, namely as Gryllotalpa sp., Polyrhachis sp., Dolichovespula sp., Apis mellifera, Periplaneta americana, Drosophila melanogaster, and one genus of Chilopoda, Scolopendra sp., were used. The samples were washed with distilled water and ۷۰% alcohol, then frozen and powdered and prepared in ۱% DMSO at eight concentrations and were evaluated for the control of lines of Gastric cancer cells /AGS, by the MTT test was performed to check the effect of doses. The affected doses of insects and centipedes were performed in ۶ replicates and three times to AGS cells. IC۵۰ and LD۵۰ were calculated by Prism version six software to check the effect of concentrations determine the effective dose and choose the best extract to control the growth of AGS /Gastric cancer cells. Results: (Pol) Polyrhachis sp. It had the best effect in controlling the AGS gastric cancer cell line. Scolopendra sp., Dolichovespula sp., Apis melifera, Gryllotalpa sp., Peripelaneta americana, were the most effective species, respectively, and finally Drosophila melanogaster was the least effect in inducing apoptosis and cell death in AGS cancer cell line. Conclusion: The results of the application of seven different genera of arthropods and insects showed that these genera have excellent therapeutic potential, particularly in the treatment of AGS cancer cells.

کلیدواژه ها:

Gastric cancer ، Centipede ، Insects ، Entomotherapy ، AGS