In Vitro Screening of Anticancer Activity of Dregea volubilis and Leptadenia reticulata Using Sulforhodamine B (SRB) Assay

The present study aimed to evaluate the anticancer potential of Dregea volubilis and Leptadenia reticulata extracts using the Sulforhodamine B (SRB) assay against selected human cancer cell lines. Ethanolic extracts of both plants were tested, and Adriamycin was used as the reference standard. The GI₅₀ values of Dregea volubilis and Leptadenia reticulata extracts were found to be ۶۲.۴ µg/mL and ۷۸.۶ µg/mL, respectively, while Adriamycin exhibited a GI₅₀ value of ۱.۲ µg/mL under similar conditions, indicating comparatively lower potency of the plant extracts. Among the two, Dregea volubilis showed better cytotoxic activity. The findings suggest that the bioactive constituents of these plants may serve as leads for the development of novel anticancer agents. This study provides the first comparative evidence of the anticancer potential of these traditional medicinal plants, supporting their ethnopharmacological relevance.

کلیدواژه ها:

Dregea volubilis ، Leptadenia reticulata ، SRB assay ، In vitro cytotoxicity ، anticancer activity ، GI₅₀