Stable Overexpression of MZF۱ Does Not Alter Proliferation, Viability, or Stemness in HEK۲۹۳T Cells

Myeloid Zinc Finger ۱ (MZF۱) is a member of the SCAN domain-zinc finger (SCAN-ZFP) transcription factor family. With a structure comprising five distinct domains, it regulates various transcriptional processes. Numerous studies suggest that MZF۱ has bi-functional roles, acting either as an oncogene or a tumor suppressor. However, its overexpression has also been associated with negligible changes in cellular functions, an aspect that remains underexplored. Additionally, although the HEK۲۹۳T cell line is widely used to investigate molecular mechanisms, the role of MZF۱ in these cells has not been reported. In this study, we assessed the effects of MZF۱ overexpression on some cellular functions in these cells. Using transposon-mediated gene transfer, we generated stably expressing cell lines and assessed their cellular functions, including cell proliferation, cell viability, and cell stemness.  Our findings show that following stable expression of MZF۱, the number of cells compared to controls did not reveal a significant difference during ۶ time-points over ۷۲ hours. Also, the MTT read absorbance, and as a consequence, cell viability did not change in the MZF۱ overexpressing cell line compared to control cells (۰.۴۹ against ۰.۴۸). By performing the colony formation assay, we did not find any significant changes in the number of colonies or the number of cells in each colony. Also, our soft agar assay did not reveal statistically significant changes in the size and number of colonies between the two groups of MZF۱-overexpressing cells and control cells. Taken together, our data provide evidence that the stable overexpression of MZF۱ does not alter cellular functions of proliferation, viability, and stemness in HEK۲۹۳T cells.

کلیدواژه ها:

MZF۱ ، HEK۲۹۳T cell line ، Cell proliferation ، Cell viability ، cell stemness