The Effect of Culture Medium Obtained from Dental Pulp Stem Cells (Conditioned Medium) on Esophageal Squamous Cell Carcinoma Cell Line (KYSE-۳۰)

Introduction: Esophageal squamous cell carcinoma (ESCC) is the eighth most prevalent cancer worldwide. Head and neck squamous cell carcinoma (HNSCC) represents over ۹۰% of all head and neck cancers, and nearly ۴۰% of patients fail treatment. Therefore, discussion of cancer treatment is essential. In this in vitro study, we examined how conditioned medium from dental pulp stem cells (DPSC-CM) influences the ESCC cell line (KYSE-۳۰).Materials and Methods: First, the middle pulp tissue of the wisdom tooth was extracted and, following sterilization, transferred to a cell culture flask containing MEM-α. After completing the culture procedures, conditioned medium (CM) was collected from the fourth passage culture after ۷۲ hours in serum-free medium. The KYSE-۳۰ cells were then treated with the CM for an additional ۷۲ hours, and cell survival was assessed using the MTT assay. Statistical significance was evaluated using one-way ANOVA followed by Tukey’s post-hoc test.Results: The results demonstrated that treatment of esophageal cancer cells with conditioned medium (CM) significantly reduced the survival rate of cancer cells compared to control samples. The treatment of KYSE-۳۰ cells with DPSC-CM led to a notable reduction in cell viability (CM group: ۵۱.۲ ± ۴.۱% vs. control group: ۷۲.۶ ± ۳.۳%; P = ۰.۰۰۴).Conclusion: DPSC-CM demonstrates the ability to reduce the proliferation of cancer cells; therefore, this medium can be considered a potential drug (therapeutic) candidate for the treatment of esophageal cancer. However, further studies are required to confirm these results.

کلیدواژه ها:

Cell Proliferation ، Mesenchymal stem cells ، Esophageal Cancer ، Conditioned medium ، In vitro study ، Dental pulp