Molecular Detection of Virulence Genes and Multi-Drug Resistance Patterns in Streptococcus Agalactiae in Clinical Bovine Mastitis: Tehran and Alborz Provinces, Iran

Streptococcus agalactiae is one of the important causes of mastitis in cows. The ability of Streptococcus agalactiae to cause disease depends on the production of a large number of virulence factors encoded by different genes. The overuse of antibiotics to treat mastitis can lead to antibiotic resistance. This research was conducted to detect selected virulence genes and assess the antibiotic resistance of Streptococcus agalactiae. For this purpose, a total of ۳۰ samples of Streptococcus agalactiae were isolated from the milk of different cows presenting clinical mastitis in Tehran and Alborz. Of these, ۲۴ samples were confirmed as Streptococcus agalactiae through the detection of the two ۱۶S-۲۳S rRNA genes.The disk diffusion method,using a panel of ۱۰ antimicrobial agents, showed a large number of strains resistant simultaneously to six antibiotics. Five virulence genes bac, bca, cylE, hylB, and cfb were screened by polymerase chain reaction (PCR). The cfb and hylB genes were found in ۹۵.۸۳ % of the isolates,while the cylE gene was detected in ۲۹.۱۶ % of the isolates.The bca and bac genes were not detected in any of the isolates. The absence of bac and bca genes suggests that they likely have minimal impact on the pathogenesis of Streptococcus agalactiae mastitis in dairy cows, while the hylB and cfb genes play a crucial role in this condition. The results presented here are one of the first molecular data concerning these five virulence genes in Streptococcus agalactiae isolates causing bovine mastitis in the Tehran and Alborz provinces , providing  a foundation for the development of diagnostic, preventive, and therapeutic methods.

کلیدواژه ها:

Antibiotic resistance ، Dairy cow ، mastitis ، Streptococcus agalactiae ، virulence genes