Expression Analysis of PVT-۱ lncRNA and TGF-β in Colorectal Cancer and Non-Cancerous Colorectal Polyps: A Case-Control Study

Colorectal cancer ranks among the most prevalent types of cancer across the globe, particularly in developing countries. Biomarkers like long non-coding RNAs (lncRNAs) have a key impact in early detection and personalized treatment. This study aimed to compare the expression levels of PVT-۱ lncRNA and TGF-β gene in colorectal cancer tissues and non-cancerous polyp tissues. Fifty colorectal cancer tissue samples and fifty non-cancerous polyp tissues, confirmed by a gastroenterologist, were analyzed. RNA was extracted, cDNA was synthesized, and expression levels of PVT-۱ lncRNA and TGF-β were quantified using real-time PCR. Data analysis was performed with SPSS software, and all experiments were conducted under identical laboratory conditions to ensure reliability. The colorectal cancer. A total of ۵۰ participants (۲۲ men and ۲۸ women) were enrolled, with a mean age of ۶۲.۵۶ ± ۱۶.۴۱ years, while the control group consisted of ۳۰ males and ۲۰ females (mean age: ۵۸.۴۱ ± ۱۱.۱۳ years). Expression analysis revealed significantly higher levels of both PVT-۱ lncRNA (fold change = X.X, P < ۰.۰۵) and TGF-β (fold change = X.X, P < ۰.۰۵) in colorectal cancer tissues compared to controls. These differences persisted despite demographic imbalances between groups. ROC curve analysis demonstrated the diagnostic potential of both biomarkers in distinguishing cancerous from non-cancerous tissues. Our findings suggest that PVT-۱ lncRNA and TGF-β may serve as promising molecular biomarkers for colorectal cancer, with potential diagnostic utility. Further validation studies are warranted to establish their role in clinical practice.

کلیدواژه ها:

Biomarker ، Colorectal cancer ، PVT-۱ lncRNA ، ROC curve ، TGF-β