Isolation and Purification of Collagen from the Skin of Black Pomfret (Parastromateus Niger) for Tissue Engineering Purpose

Collagen is one of the main biomaterials with tissue engineering application. It plays an important role in designing and providing scaffold to support the interactions between cells and scaffolds aiming for high attachment, proliferation and differentiation. Currently research proceed to extraction collagen from the skin Black pomfret (Parastromateusniger), one of the most important and economical species of the Oman sea and Persian gulf. Collagen is the most abundant protein in vertebrates; have a biomedical matters, pharmacutical, tissue engineering, industrial and biotechnological application. Samples taken from the fishery market and carried out to laboratory. Acid soluble collagen (ASC) isolated by acidic methods at the ۴℃ for ۵ day. Then, collagen precipitates with NaCl and purification to ۰.۱M acetic acid solution and distilled water. The results showed that collagen this fish is highest value of ۱۳.۶% on a dry weight basis. The SDS-PAGE pattern show the ASC producted from this species have hetrotrimer structure and made of two chains; α۱and α۲ . So collagen provides is considered type I collagen. Moreover UV-spectra exhibited this collagen have a absorbance ۲۲۰nm. Therefore black pomfret fish can be used for alternative collagen source. It may also be subsequently increase the commercial value of fish.Collagen is one of the main biomaterials with tissue engineering application. It plays an important role in designing and providing scaffold to support the interactions between cells and scaffolds aiming for high attachment, proliferation and differentiation. Currently research proceed to extraction collagen from the skin Black pomfret (Parastromateusniger), one of the most important and economical species of the Oman sea and Persian gulf. Collagen is the most abundant protein in vertebrates; have a biomedical matters, pharmacutical, tissue engineering, industrial and biotechnological application. Samples taken from the fishery market and carried out to laboratory. Acid soluble collagen (ASC) isolated by acidic methods at the ۴℃ for ۵ day. Then, collagen precipitates with NaCl and purification to ۰.۱M acetic acid solution and distilled water. The results showed that collagen this fish is highest value of ۱۳.۶% on a dry weight basis. The SDS-PAGE pattern show the ASC producted from this species have hetrotrimer structure and made of two chains; α۱and α۲ . So collagen provides is considered type I collagen. Moreover UV-spectra exhibited this collagen have a absorbance ۲۲۰nm. Therefore black pomfret fish can be used for alternative collagen source. It may also be subsequently increase the commercial value of fish.

کلیدواژه ها:

Black pomfret ، skin ، Collagen ، Biomaterial