Design and manufacturing of novel smart pegylated nano-liposome co-encapsulating two herbal compounds: Silibinin and Glycyrrhizic acid with HAb۱۸ monoclonal antibody for targeted co-delivery to liver cancer cells

Objective(s): Herbal nano-liposomes, also referred to as nano-Phytosomes, are formed through hydrogen bonding interactions between the phospholipids of lipid membranes and phytomolecules. This structure enhances the delivery efficiency of therapeutic agents. This study focuses on the use of PEGylated nano-liposomes co-loaded with two anti-cancer compounds derived from herbs, silibinin and glycyrrhizic acid, to target liver cancer cells.Materials and Methods: The co-encapsulated nanoscale liposomes were synthesized using the thin-layer film hydration method with HEPES buffer, followed by sonication. The vesicles encapsulating silibinin and glycyrrhizic acid consisted of DPPC, cholesterol, and DSPE-mPEG۲۰۰۰ in a molar percentage ratio of approximately ۶۱.۵:۳۵:۳.۵. A fluorescent label (DIL) was incorporated into the lipid bilayer at a concentration of ۰.۱ mol%. The multilamellar vesicles were then sonicated and filtered to produce the nano-liposomes. To enhance targeting, these co-encapsulated the nano-liposomes were conjugated with the monoclonal antibody HAb۱۸. The resulting PEGylated nano-liposome formulation demonstrated a narrow size distribution, with an average particle diameter of approximately ۴۵ nm. The zeta potential of the co-encapsulated nano-Phytosome was measured at -۲۳.۲۵mV. The encapsulation efficiencies of silibinin and glycyrrhizic acid were approximately ۲۴.۳۷% and ۶۸.۷۸%, respectively.Results: The study revealed that the mean diameter of the nano-Phytosome increased upon targeting. Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) images confirmed that the average diameters of the targeted co-encapsulated nano-liposomes were approximately ۸۴ nm and ۸۱ nm, respectively, while the monoclonal antibody HAb۱۸ exhibited an average diameter of around ۱۶.۱ nm and ۱۵ nm.Conclusion: This targeted nano-system offers efficient delivery of herbal drugs to liver cancer cells.

کلیدواژه ها:

Targeting ، Nano-liposome ، Silibinin ، Glycyrrhizic acid ، Liver cancer