Dose-Dependent Effects of Intratesticular Adipose-Derived Mesenchymal Stem Cell Injection on Heat-Induced Spermatogenesis Disorder in Wistar Rats: Focus on Apoptosis and Oxidative Stress

Background & Objective: Spermatogenesis is a temperature-dependent process, and testicular heat stress can cause spermatogenic failure by inducing cell apoptosis and oxidative stress, ultimately leading to male infertility. Adipose-derived mesenchymal stem cells (AMSCs) have been considered an effective therapy for various tissue degenerations, demonstrating the ability to stimulate testicular regeneration and restore spermatogenesis. The current study focuses on the therapeutic potential of AMSCs on semen quality, testicular morphological changes, and oxidative stress parameters in rats exposed to heat stress.Methods: In this experimental study, ۳۵ adult male rats were randomly assigned to five groups: Group I (control), Group II (vehicle), Group III (heat stress group, temperature-humidity index: ۴۳ °C for ۲۰ minutes), and Groups IV and V (treatment groups receiving ۰.۵×۱۰⁶ and ۱×۱۰⁶ AMSCs, respectively, on the second and fifteenth days after heat stress induction). Sixty days after heat stress exposure, the animals were euthanized; serum testosterone levels and oxidative stress biomarkers were analyzed, and the testes and epididymis were collected for histological and sperm evaluation.Results: Scrotal heat stress caused deleterious effects on testicular histological structure and function. Testosterone levels and total antioxidant capacity were significantly reduced in the heat stress group. The ۱×۱۰⁶ AMSCs-treated group showed moderately preserved testicular tissue morphology. Apoptotic spermatogonia and primary spermatocytes decreased significantly in the AMSCs treatment groups in a dose-dependent manner. Malondialdehyde levels and total antioxidant capacity were also improved. Progressive sperm motility, sperm count, and viability were notably enhanced in the AMSCs-treated groups.Conclusion: A single dose-dependent injection of AMSCs demonstrated regenerative properties that improved with increasing cell number. Overall, administration of ۱×۱۰⁶ AMSCs can alleviate testicular damage and promote the spermatogenesis process in testicular hyperthermia.

کلیدواژه ها:

Mesenchymal stem cells ، Adipose tissue ، Testis ، Hyperthermia ، Spermatogenesis ، Oxidative stress ، Animal study