The effect of training and Tribulus terrestris extract on the antioxidant system and telomere functional markers in the liver tissue of rats exposed to stanozolol

Objective: The present study aimed to assess the effect of training along with administration of Tribulus terrestris (T) on the antioxidant system and telomere functional markers in the liver tissue of rats exposed to stanozolol.Materials and Methods: Forty- nine male rats, with average age and weight of ۸-۱۰ weeks and ۱۸۰-۲۲۰ g respectively, were randomly divided into ۷ groups of seven rats: ۱) Sh: Sham, ۲) S: stanozolol, ۳) S+T۵۰: stanozolol+ ۵۰ mg/kg T. terrestris, ۴) S+T۱۰۰: stanozolol+ ۱۰۰ mg/kg T. terrestris, ۵) S+RT: stanozolol + resistance training, ۶) S+RT+T۵۰: stanozolol + resistance training + ۵۰ mg/kg T. terrestris, and ۷) S+RT+T۱۰۰: stanozolol + resistance training + ۱۰۰ mg/kg T. terrestris. Rats in the S groups received ۵ mg/kg stanozolol intraperitoneally (۲۵ mg/kg/wk). Groups ۵ (R+T), ۶ (S+RT+T۵۰), and ۷ (S+RT+T۱۰۰) performed resistance trainings three sessions per week with an intensity of ۳۰-۱۰۰ percent of body weight for eight weeks. Also, groups ۳ (S+T۵۰), ۴ (S+T۱۰۰), ۶ (S+RT+T۵۰) and ۷ (S+RT+T۱۰۰) received daily ethanolic extract of T with doses of ۵۰ and ۱۰۰ mg/kg intraperitoneally.Results: In the S+RT, S+T۵۰, S+T۱۰۰, S+RT+T۵۰, and S+RT+T۱۰۰ groups, malondialdehyde (MDA) levels were significantly lower and gene expression of phosphoinositide ۳-kinases (PI۳K), protein kinase B (Akt), and telomerase reverse transcriptase (TERT) levels were higher than the S group. Also, phosphatase and tensin homolog (PTEN) and TERT gene expression levels in the S+T۱۰۰ group were significantly higher than the S+T۵۰ group.Conclusion: Training and T have a positive effect on the transcription pathway of antioxidants and telomere protection.

کلیدواژه ها:

Exercise ، Tribulus terrestris ، Oxidative stress ، Telomere Function ، Liver ، Stanozolol