Rapid analysis and separation of fifteen beta-lactam drugs Using Reversed-Phase High-Performance Liquid Chromatography

Beta-lactam medications are among the commonly used antibiotics. These drugs kill germs by disrupting cell wall formation. This drug treats bacterial infections in numerous body sections. A new, quick, high-performance liquid chromatography approach has been developed and verified by the FDA and EMA for the simultaneous estimation of drugs in their medicinal and pure forms. This study deals with the determination of beta-lactam drugs (Amoxicillin, Ampicillin, Cephalexin, Cefotaxime, Cefoxitin, Cefamandole, Cephalothin, Piperacillin, Penicillin, Oxacillin, Cloxacillin, Nafcillin, Carbenicillin, Mezlocillin, Dicloxacillin) by a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) technique based on an UV detector using a column NEUCLEODUR C-۱۸ (۴.۰ mm × ۱۰۰ mm, ۵µm particle size), The heat of the chromatographic column was ۳۰oC, and the mobile phase of acetonitrile and KH۲PO۴ using a progression elution with a total separation time of ۱۹.۵۴ minutes, The buffer solution was pH ۴.۵, and the wavelength was ۲۲۰ nm. The concentration range was from ۰.۲ to ۲۰ µg mL-۱, with coefficients of determination (R۲) of ۰.۹۹۹۴. Recoveries ranged from ۹۰.۸۶% to ۱۰۰.۴%. The limit of detection (LOD) and limit of quantitation (LOQ) were ۰.۰۰۶۹ to ۰.۰۶۳۹ µg mL-۱ and ۰.۰۲۱۱ to ۰.۱۹۹۷ µg mL-۱, respectively. In this study, the chromatographic method was used to simultaneously identify drugs in medicinal dosage forms obtained from pharmacies.

کلیدواژه ها:

Analysis ، Beta lactam drugs ، Amoxicillin ، Carbenicillin ، Dicloxacillin ، Reversed-Phase High-Performance Liquid Chromatography