Cytotoxicity and Antibacterial Activity of Ag@MOF-۵ Loaded Alginate–Gelatin Hydrogel as a Promising Antimicrobial Biomaterial

The development of safe multifunctional biomaterials that possess antibacterial activity is of great importance. In this study, a composite alginate–gelatin hydrogel incorporating silver-modified MOF-۵ was developed to enhance antibacterial activity. The successful synthesis of the samples was confirmed by XRD characterization of MOF-۵, showing characteristic peaks at ۲θ = ۶.۸°, ۹.۶°, and ۱۳.۷°, which remained identifiable after modification with Ag. FTIR analysis of the Hy/Ag(۲۰)@MOF-۵ sample indicated an interaction between the carboxylate groups (–COO–) of alginate and the amino groups (–NH₂) of gelatin, with the absorption band shifting from ۱۵۸۳ cm⁻¹ to ۱۵۴۵ cm⁻¹, signifying the incorporation of Ag@MOF-۵ within the hydrogel matrix. SEM-EDX results of Hy/Ag(۲۰)@MOF-۵ revealed a uniformly porous surface structure and confirmed the presence of Zn, O, C, and Ag elements. Ag@MOF-۵ in alginate-gelatin hydrogel causes a decrease in the swelling ratio and biodegradability percentage. The release profile of Ag indicates that in the Hy/Ag(۲۰)@MOF-۵ sample, the concentration of released Ag is higher at ۷.۴ µg/mL after ۲۴ hours; however, the release of Ag ions remains controlled. The cytotoxicity test, shows a cell viability of over ۷۹ % across all sample types, categorizing them as non-toxic within the tested concentration range. The antibacterial activity test results indicate that the hy/Ag(۲۰)@MOF-۵ sample exhibits the highest antibacterial activity against Staphylococcus aureus and Escherichia coli, with MIC values of ۱.۲ ± ۰.۱ and ۱.۰ ± ۰.۱ mg/mL, respectively, and MBC values of ۲.۵ ± ۰.۱ and ۲.۷ ± ۰.۲ mg/mL, and is capable of killing all bacteria within ۲۴ hours.

کلیدواژه ها:

Hydrogel ، MOF-۵ ، Ag ، antibacterial ، Cytotoxicity ، alginate ، Gelatin