The comparison between HIIT and Resistance Training in muscle expression of FTO and PPAR-γ in obese diabetic rats

Objective: According to the World Health Organization (WHO), the prevalence of overweight and obesity among adults aged ۱۸ and above is estimated to be around ۲ billion worldwide. The purpose of this study was to investigate the comparison between High Intensity Interval Training and Resistance Training in expression of FTO and PPAR-γ genes in muscle tissue among type ۲ diabetic obese rats.Methods: This experimental study utilized a sample of ۱۲ male Wistar rats, with an average bodyweight of ۲۲۰±۲۰ g and an age of ۱۰ weeks. The rats were subjected to a high-fat diet for a duration of six weeks. To induce type ۲ diabetes, a single intraperitoneal injection of ۳۰ mg/kg of freshly prepared streptozotocin (STZ) was administered, dissolved in citrate buffer with a pH of ۴.۵. The rats with diabetes were then randomly divided into three groups: HIIT, Resistance training. Measurements were taken ۴۸ hours after the final training session. The expression level of FTO and PPAR-γ in the muscles were assessed using the real-time PCR method. Statistical analysis was conducted using independent samples t-test and Analysis of Covariance (ANCOVA) to compare the means between the groups.Results: FTO expression decreased significantly in the HIIT group (P<۰.۰۴۲) while, the RT group faced no significant changes (P<۰.۴۵۲). However, PPAR-γ expression increased only in the HIIT group (P<۰.۰۲۰). The were no significant differences between the changes of FTO and PPAR-γ expression between groups (FTO P<۰.۲۴۳ and PPAR-γ P<۰.۱۵۸).Conclusion: the findings from this study suggest that short-term and high-intensity aerobic exercises have a more pronounced impact on the expression of FTO and PPAR-γ genes, as well as related factors, in individuals with obesity and type ۲ diabetes compared to resistance exercises. The aerobic exercises demonstrated significant alterations in the expression of both the FTO and PPAR-γ genes.Objective: According to the World Health Organization (WHO), the prevalence of overweight and obesity among adults aged ۱۸ and above is estimated to be around ۲ billion worldwide. The purpose of this study was to investigate the comparison between High Intensity Interval Training and Resistance Training in expression of FTO and PPAR-γ genes in muscle tissue among type ۲ diabetic obese rats. Methods: This experimental study utilized a sample of ۱۲ male Wistar rats, with an average bodyweight of ۲۲۰±۲۰ g and an age of ۱۰ weeks. The rats were subjected to a high-fat diet for a duration of six weeks. To induce type ۲ diabetes, a single intraperitoneal injection of ۳۰ mg/kg of freshly prepared streptozotocin (STZ) was administered, dissolved in citrate buffer with a pH of ۴.۵. The rats with diabetes were then randomly divided into three groups: HIIT, Resistance training. Measurements were taken ۴۸ hours after the final training session. The expression level of FTO and PPAR-γ in the muscles were assessed using the real-time PCR method. Statistical analysis was conducted using independent samples t-test and Analysis of Covariance (ANCOVA) to compare the means between the groups. Results: FTO expression decreased significantly in the HIIT group (P<۰.۰۴۲) while, the RT group faced no significant changes (P<۰.۴۵۲). However, PPAR-γ expression increased only in the HIIT group (P<۰.۰۲۰). The were no significant differences between the changes of FTO and PPAR-γ expression between groups (FTO P<۰.۲۴۳ and PPAR-γ P<۰.۱۵۸). Conclusion: the findings from this study suggest that short-term and high-intensity aerobic exercises have a more pronounced impact on the expression of FTO and PPAR-γ genes, as well as related factors, in individuals with obesity and type ۲ diabetes compared to resistance exercises. The aerobic exercises demonstrated significant alterations in the expression of both the FTO and PPAR-γ genes.

کلیدواژه ها:

Aerobic ، T۲D ، Interval ، Mice ، Glucose ، HIIT ، FTO ، PPARy