Exercise Appears to Promote Lifespan by Regulating Aging Biomarkers TAF۱۵ and HKDC۱

Introduction Aging is a multifaceted process at the biological level that involves cellular senescence, oxidative stress, and chronic inflammation. These components contribute to a decline in physiological function and increased vulnerability to age-related diseases. This study investigates the influence of exercise on two key biomarkers of aging, TAF۱۵, and HKDC۱, through the comparison of their expressions between athletes and non-athletes.Materials/Methods: Blood samples were obtained from ۳۰ individuals, including ۱۵ athletes and ۱۵ non-athletes. Total RNA was extracted, after which cDNA synthesis was conducted to investigate the expression of the HKDC۱ and TAF۱۵ genes. The expression levels of both genes were subsequently determined using qRT-PCR. Indeed, the results of HKDC۱ expression presented ۱.۴۴۵-fold higher in athletes compared to healthy individuals, with the statistical significance level of p = ۰.۰۰۷, indicating its use in glucose metabolism and further energy balance. On the contrary, TAF۱۵ mRNA expressed a ۱.۱۵۹-fold increase in athletes; however, the difference did not show statistical significance at the level of p = ۰.۱۰۴.Conclusion: Data herein presented indicate that exercise regularly improves metabolic health and may prevent an age-related decline in metabolic health via the regulation of HKDC۱ and TAF۱۵. The current study reinforces the role of physical activity as an important therapeutic strategy in healthy aging and the prevention of age-related diseases, and further research is warranted to explain the mechanisms of these biomarkers to exercise.Introduction Aging is a multifaceted process at the biological level that involves cellular senescence, oxidative stress, and chronic inflammation. These components contribute to a decline in physiological function and increased vulnerability to age-related diseases. This study investigates the influence of exercise on two key biomarkers of aging, TAF۱۵, and HKDC۱, through the comparison of their expressions between athletes and non-athletes.Materials/Methods: Blood samples were obtained from ۳۰ individuals, including ۱۵ athletes and ۱۵ non-athletes. Total RNA was extracted, after which cDNA synthesis was conducted to investigate the expression of the HKDC۱ and TAF۱۵ genes. The expression levels of both genes were subsequently determined using qRT-PCR. Indeed, the results of HKDC۱ expression presented ۱.۴۴۵-fold higher in athletes compared to healthy individuals, with the statistical significance level of p = ۰.۰۰۷, indicating its use in glucose metabolism and further energy balance. On the contrary, TAF۱۵ mRNA expressed a ۱.۱۵۹-fold increase in athletes; however, the difference did not show statistical significance at the level of p = ۰.۱۰۴.Conclusion: Data herein presented indicate that exercise regularly improves metabolic health and may prevent an age-related decline in metabolic health via the regulation of HKDC۱ and TAF۱۵. The current study reinforces the role of physical activity as an important therapeutic strategy in healthy aging and the prevention of age-related diseases, and further research is warranted to explain the mechanisms of these biomarkers to exercise.

کلیدواژه ها:

Exercise ، Aging Biomarkers ، TAF۱۵ ، HKDC۱