Comparative Effects of Bioglass and Zinc-Doped Bioglass on VEGF and FLT۱ Gene Expression in Wound Healing

IntroductionIt is the most extensive organ in the body, providing a critical barrier function for the prevention of infections and against various environmental insults. The process of wound healing is very active and complicated, with numerous variables that include, among others, the extent of injury, blood flow, and immune condition. Improvements within tissue engineering have enabled the fabrication of scaffolds to support wound healing by providing cells with surfaces for attachment, migration, and proliferation. Of note, the use of bioactive materials such as bioglass and zinc in collagen-based scaffolds represents an exciting area of recent research that has shown promise in enhancing tissue regeneration.Methods and Materials:In this context, the functional activity of BG, ZBG-integrated polyurethane (PU), polycaprolactone (PCL), collagen, and BG scaffolds was evaluated on wound healing. For this, multilayered scaffolds were fabricated to ensure controlled release of the bioactive molecules. RT-PCR for the two major angiogenic factor genes, VEGF and FLT۱, were expressed as proofs for the vascularization potency of the scaffolds. In addition, full-thickness wound models in rats were used in an in vivo wound healing experiment.Results:Results indicated that scaffolds containing bioactive glass exhibited a significantly higher wound healing effect through higher VEGF and FLT۱ expression compared with the control. Adding zinc to this bioglass enhanced these wound-healing properties: inducing fibroblast proliferation, antibacterial activity, and angiogenesis. To develop mechanically stable and biocompatible scaffolds, the combination of PU and PCL with collagen can support cell adhesion, proliferation, and regeneration of the extracellular matrix.Conclusion:This study investigates the translational potential of PU-PCL/collagen bioglass-based scaffolds, with specific emphasis on the role of zinc-enhanced scaffolds, in accelerating the wound healing process through promoting angiogenesis and cellular responses associated with tissue regeneration. Therefore, the results provided here justify the pre clinical application of multilayer scaffold in advanced wound management and offer a promising strategy for enhanced healing in skin-injured patients.Keywords: VEGF, FLT۱, Multilayer Bioglass Scaffold, Wound HealingIntroductionIt is the most extensive organ in the body, providing a critical barrier function for the prevention of infections and against various environmental insults. The process of wound healing is very active and complicated, with numerous variables that include, among others, the extent of injury, blood flow, and immune condition. Improvements within tissue engineering have enabled the fabrication of scaffolds to support wound healing by providing cells with surfaces for attachment, migration, and proliferation. Of note, the use of bioactive materials such as bioglass and zinc in collagen-based scaffolds represents an exciting area of recent research that has shown promise in enhancing tissue regeneration. Methods and Materials:In this context, the functional activity of BG, ZBG-integrated polyurethane (PU), polycaprolactone (PCL), collagen, and BG scaffolds was evaluated on wound healing. For this, multilayered scaffolds were fabricated to ensure controlled release of the bioactive molecules. RT-PCR for the two major angiogenic factor genes, VEGF and FLT۱, were expressed as proofs for the vascularization potency of the scaffolds. In addition, full-thickness wound models in rats were used in an in vivo wound healing experiment. Results:Results indicated that scaffolds containing bioactive glass exhibited a significantly higher wound healing effect through higher VEGF and FLT۱ expression compared with the control. Adding zinc to this bioglass enhanced these wound-healing properties: inducing fibroblast proliferation, antibacterial activity, and angiogenesis. To develop mechanically stable and biocompatible scaffolds, the combination of PU and PCL with collagen can support cell adhesion, proliferation, and regeneration of the extracellular matrix. Conclusion:This study investigates the translational potential of PU-PCL/collagen bioglass-based scaffolds, with specific emphasis on the role of zinc-enhanced scaffolds, in accelerating the wound healing process through promoting angiogenesis and cellular responses associated with tissue regeneration. Therefore, the results provided here justify the pre clinical application of multilayer scaffold in advanced wound management and offer a promising strategy for enhanced healing in skin-injured patients.Keywords: VEGF, FLT۱, Multilayer Bioglass Scaffold, Wound Healing