Role of miRNA-۱۴۵ in diagnosis and treatment of Breast Cancer

Cancer is still one of the most important challenges to health worldwide, with an ever-increasing incidence rate for different kinds of cancers, especially respiratory and colorectal cancers. While early diagnosis and appropriate treatment are imperative, conventional therapies mostly suffer from a lack of specificity and resulting side effects. microRNAs have emerged as critical regulators of gene expression and potential therapeutic target in cancer. The current study has emphasized miR-۱۴۵, a well-established tumor suppressor whose expression is reportedly downregulated in the majority of malignancies, including breast cancer. We investigate here the functionality of miR-۱۴۵ in regulating oncogenes due to their involvement in tumor progression. A combined approach of different molecular techniques has been employed in the study for analyzing the expression levels of miR-۱۴۵ and their interaction with target genes such as fascin-۱, c-myc, SMAD۲/۳, and IGF-۱R. Here, we report that miR-۱۴۵ significantly decreases the proliferative and migratory capability of breast cancer models both in vitro and in vivo, especially in combination with classic chemotherapy agents such as ۵-fluorouracil. We further investigate the translational potential of miR-۱۴۵ delivery using adenoviral vectors and point out the potential for increasing anti-tumor effects. The present study will further describe miR-۱۴۵ in regulating breast cancer and its potential as a biomarker and therapeutic target in depth and thereby provide contributions to the advance of miRNA-based strategies for cancer therapy.Cancer is still one of the most important challenges to health worldwide, with an ever-increasing incidence rate for different kinds of cancers, especially respiratory and colorectal cancers. While early diagnosis and appropriate treatment are imperative, conventional therapies mostly suffer from a lack of specificity and resulting side effects. microRNAs have emerged as critical regulators of gene expression and potential therapeutic target in cancer. The current study has emphasized miR-۱۴۵, a well-established tumor suppressor whose expression is reportedly downregulated in the majority of malignancies, including breast cancer. We investigate here the functionality of miR-۱۴۵ in regulating oncogenes due to their involvement in tumor progression. A combined approach of different molecular techniques has been employed in the study for analyzing the expression levels of miR-۱۴۵ and their interaction with target genes such as fascin-۱, c-myc, SMAD۲/۳, and IGF-۱R. Here, we report that miR-۱۴۵ significantly decreases the proliferative and migratory capability of breast cancer models both in vitro and in vivo, especially in combination with classic chemotherapy agents such as ۵-fluorouracil. We further investigate the translational potential of miR-۱۴۵ delivery using adenoviral vectors and point out the potential for increasing anti-tumor effects. The present study will further describe miR-۱۴۵ in regulating breast cancer and its potential as a biomarker and therapeutic target in depth and thereby provide contributions to the advance of miRNA-based strategies for cancer therapy.