Cinnamophilin ameliorates testosterone-induced prostatic hyperplasia and fibrosis by regulating ۵α-reductase and TGF-β/Smad signaling pathway

Objective(s): Androgen and TGF-β۱/Smad signaling pathways play important roles in epithelial-mesenchymal transition (EMT), fibrosis, and the development of benign prostatic hyperplasia (BPH). Cinnamophilin is extracted from Cinnamomum philippinense. The anti-proliferative and anti-fibrosis effects of cinnamophilin on the prostate remain unclear. This study aimed to investigate the therapeutic effects and molecular mechanism of action of cinnamophilin on prostate growth in testosterone propionate (TP)-treated mice.Materials and Methods: The study was conducted both in vivo and in vitro. TP was injected subcutaneously to induce prostate enlargement and growth. Cinnamophilin (۴۰ mg/kg) was orally administered once a day in TP (۷.۵ mg/ kg)-treated mice for ۲۸ days. The morphological characteristics and fibrosis of the prostate were examined by H&E (Hematoxylin and Eosin) and Masson’s trichrome stain. Protein expression was determined by Western blot. BPH-۱ and WPMY-۱ cells were treated with different concentrations of cinnamophilin (۱–۱۰۰ μM). Results: Cinnamophilin (۴۰ mg/kg) significantly reduced prostate weight and prostate index in animal models. Cinnamophilin inhibited the protein expression of ۵α-reductase type II and prostate-specific antigen (PSA) in TP-treated mice. Cinnamophilin reversed morphological changes, EMT, and fibrosis in TP-treated mice. Cinnamophilin increased E-cadherin but decreased N-cadherin, vimentin, fibronectin, α-SMA, TGFBR۲, TGF-β۱, p-Smad۲/۳, collagen I, collagen III, and collagen IV protein expressions. The expression of Smad۲/۳ was not significantly different among these groups. Cinnamophilin (۱۰۰ μM) inhibited proliferation at ۴۸ hr in BPH-۱ and WPMY-۱ cells. Conclusion: These findings suggest that cinnamophilin inhibits prostate growth and mitigates EMT and fibrosis by regulating TGFβ/Smad signaling pathways.

کلیدواژه ها:

Cinnamophilin ، EMT ، Fibrosis ، Prostate hyperplasia ، Smad ، TGF-β