The antibacterial properties of novel nanocomposite films of chitosan and phosphoramide with different percentages of magnetic nanoparticles

A novel nanocomposite films of chitosan, phosphoramid and magnetic (Fe3O4) nanoparticles was prepared by blending phosphoramide and magnetic nanoparticles with chitosan solution folloed by ultrasonic technique. Iron oxide nanoparticles (Fe3O4) were synthesized by reaction of iron nitrate and iron chloride and starch as a capping agent. The magnetic nanoparticles were investigated with the use of a strong magnetic field. The phosphoramide compound was synthesized from the reaction of morpholine with N-4-nitrophenyl phosphoramidic dichloride. The nanocomposite films prepared with chitosan, phosphoramide, 2 and 3% of Fe3O4 NPs. The prepared nanocomposite films were characterized using techniques such as Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and X-ray diffraction (XRD). The swelling studies were also performed in PBS. The antibacterial activities of nanocomposite films were tested against the bacterial species Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative). Results revealed greater antibacterial effects of the films against Gram-positive bacteria. Also, nanocomposite films containing higher percent of magnetite nanoparticles showed more antibacterial activities.

کلیدواژه ها:

Nanocomposite ، Chitosan ، Fe3O4 nano particles ، Phosphoramide