Research Article: Dietary effects of ascorbic acid and cobalt chloride on some biological parameters and gene expression in Acipenser baerii

This research investigated the effects of dietary cobalt chloride (CoCl۲) and ascorbic acid (AA) on growth, physiological, and molecular parameters in Siberian sturgeon (Acipenser baerii). Two hundred seventy juveniles were divided into eight groups fed diets containing ۲ or ۴ mg/kg CoCl۲ and ۲۰۰ or ۸۰۰ mg/kg AA for ۱۲ weeks. Results showed significant differences in final weight, length, weight gain, and growth rate among treatments (p<۰.۰۵), indicating differences when compared to the control group. However, no significant difference was observed in specific growth rate, condition factor, Average daily growth, or protein efficiency ratio compared to the control (p>۰.۰۵). The lowest and highest AA/cobalamin levels were found in the control and the ۴ mg/kg CoCl۲ with ۲۰۰ mg/kg AA, and the ۲ mg/kg CoCl۲ groups, respectively (p<۰.۰۵). Maximum serum iron level was observed in fish fed ۸۰۰ mg/kg CoCl۲, and the lowest glucose was in the control (p<۰.۰۵). The cortisol level was significantly higher in ۲ mg/kg CoCl۲ with ۲۰۰ mg/kg AA group compared to the control, which had the lowest levels (p<۰.۰۵). The highest hsp۷۰ and p۴۵۰ gene upregulation, as well as the highest GH and igf-۱ expression, occurred in the ۴ mg/kg CoCl۲ with ۲۰۰ mg/kg AA group, with the lowest expression in the control (p<۰.۰۵). Overall, ۴ mg/kg CoCl۲ and ۲۰۰ mg/kg AA supplementation had more positive effects on growth performance, hematological and biochemical indices, and the modulation of genes associated with growth, stress response, and immunity in Siberian sturgeon.

کلیدواژه ها:

Micronutrients ، Growth ، Physiological indices ، Gene expression ، Acipenser baerii