Fabrication of Pistacia atlantica Desf. Oil-Encapsulated Chitosan Nanoparticles in Polyvinyl Alcohol Nanofibers: Biocompatibility and Antioxidant Properties for Skin Care Applications

This study provides a comprehensive nutritional and phytochemical analysis of Pistacia atlantica Desf. fruit (PAF), which revealed a high fat content (۳۳.۹۰%) and carbohydrate content (۶۲.۴۱%), identifying it as a significant energy source. Phytochemical profiling demonstrated notable levels of phenolics (۱۷.۷۰ ± ۱.۵۹ mg GAE/g dry weight) and flavonoids (۶.۴۶ ± ۰.۳۵ mg CE/g), contributing to substantial antioxidant properties. A novel nanocomposite was developed by encapsulating Pistacia atlantica oil (PAO) within chitosan nanoparticles (CNPs) using ionic gelation with sodium tripolyphosphate, followed by embedding these nanoparticles into polyvinyl alcohol (PVA) nanofibers. Characterization of the resulting nanocomposite revealed a mean particle size of ۱۰۴.۷۶ nm and a polydispersity index (PDI) of ۰.۴۱, indicating a uniform size distribution. The encapsulation efficiency (EE%) of PAO was consistent across formulations, while the loading capacity (LC%) increased with higher CNP content, achieving a maximum of ۳۲.۳۷% for a PVA ratio of ۱:۲. Antioxidant assays confirmed robust activity, with FRAP and DPPH values of ۷۹.۴ ± ۲.۱ µmol ESF/۱۰۰g and an IC₅₀ of ۷۳۴۴.۷ µg/mL, respectively. Cell viability assays demonstrated excellent biocompatibility, with CNP and PVA-CNP formulations maintaining over ۸۲% viability in human dermal fibroblast cells. The CNP-PVA nanofiber system infused with PAO demonstrates controlled release, robust antioxidant properties, and superior biocompatibility, positioning it as a potential solution for wound healing and skin care needs

کلیدواژه ها:

Pistacia atlantica ، Chitosan nanocapsules ، PVA nanofibers ، Antioxidant ، WOUND HEALING ، Biocompatibility