Therapeutic effect of hydroalcoholic extract of Moringa oleifera and sulfurphane on melanoma caused by B۱۶f۱۰ cell line in mouse model C۵۷bl/۶

Background: Moringa oleifera, an herbal medicine, has been found to possess anti-tumor effects against various cancers. Objectives: The aim of this study was to investigate the anti-inflammatory and antioxidant properties of Moringa oleifera plant extract on metastasis. Methods: Initially, the presence of phenolic compounds in Moringa oleifera was confirmed through HPLC and spectrophotometer methods. Following that, the anticancer effects of the phenolic chemicals sulforaphane and Moringa oleifera on B۱۶F۱۰ cancer cells were investigated. Following this, melanoma tumors were induced in C۵۷BL۶ mice using the B۱۶F۱۰ cell line, and the mice were treated with the extract. The extract was identified to contain quercetin, gallic acid, and caffeic acid. Results: The rate of cell death observed in the sulforaphane-exposed cell group was lower compared to the group treated with Moringa oleifera. Furthermore, tumor volume decreased significantly after one and two weeks of therapy with all three doses of Moringa extract or phenolic chemicals (P<۰.۰۰۱). Notably, the tumor volume decreased the most with the ۰.۰۲ g dose compared to the other two doses (P <۰.۰۰۱). Conclusion: This study demonstrates the positive effect of antioxidants present in Moringa oleifera extract on reducing the viability of cancer cells and tumor volume in mice. Further research is recommended to establish the potential of Moringa oleifera as an effective anticancer drug with greater certainty. Additionally, it is advisable to conduct studies on human subjects as well.

کلیدواژه ها:

Moringa oleifera ، sulforaphane ، Melanoma ، B۱۶F۱۰ melanoma cell line