Synergistic effects of hyaluronic acid and azelaic acid in cutaneous wound healing: A histopathological study

Cutaneous wounds pose significant clinical challenges by disrupting the body’s protective barrier, necessitating optimized healing strategies to improve patient outcomes. Hyaluronic acid (HA) facilitates tissue repair, while azelaic acid (AzA) exhibits anti-inflammatory and antibacterial properties. This study evaluated the synergistic effects of topical HA and AzA on wound healing in a rabbit model. Standardized full-thickness wounds (۶ mm diameter, ۲ mm depth) were created using a skin punch. Treatments with HA and AzA were applied every ۱۲ hours for ۱۴ days, while control wounds received a placebo solution. Tissue samples were collected on days ۷ and ۱۴ for histopathological examination using hematoxylin & eosin and Masson's trichrome staining. The treatment group showed complete epidermal coverage, well-organized granulation tissue, enhanced collagen deposition, and reduced inflammation compared to controls, which exhibited incomplete regeneration and disorganized cellular structures. These findings demonstrate that combined topical application of HA and AzA significantly accelerates cutaneous wound healing, highlighting its potential clinical utility for improved tissue repair.

کلیدواژه ها:

Rabbit ، Hyaluronic Acid ، Azelaic Acid ، Wound Healing ، Pathology