Ameliorative effects of Corchorus olitorius leaf ethanolic extract on letrozole-induced polycystic ovary syndrome (PCOS) in rats

Objective: To evaluate the effects of Corchorus olitorius leaf extract on induced polycystic ovarian syndrome (PCOS) rats.Materials and Methods: ۳۶ female Wistar rats were divided into six groups (n=۶) including Sham, PCOS + vehicle (Dimethyl sulfoxide), PCOS + Clomiphene citrate, PCOS + ۲۰۰, ۴۰۰, and ۶۰۰ mg/kg Corchorus olitorius. The Sham group was administered CMC (carboxymethylcellulose) (۰.۵%) ۱ ml/۰.۱ kg, while the PCOS groups were administered letrozole (۱ mg/kg) dissolved in ۰.۵% CMC solution for ۲۱ days via oral gavage. The PCOS condition was established to be successful when Papanicolaou-stained vaginal cytology of days ۱۳-۲۱ showed a greater dominance of clusters of empty cornified squamous cells. The duration of treatments was for ۱۴ days ( via oral gavage) and euthanization occurred on the ۱۵th day. Hormonal levels, glucose level, lipid profile, hematological indices, liver function, kidney function, and histopathological studies of the ovaries and uteri were all determined.Results: PCOS induction led to abnormalities in hormonal levels, lipid profile, glucose levels, ovarian morphology, uterine morphology, and vaginal cytology of the PCOS rats compared to the Sham group (p<۰.۰۵). C. olitorius leaf extract showed its ameliorative effects in terms of normalizing the altered vaginal cytology, restoring most parameters, and improving the appearance of ovarian and uterine morphology.Conclusion: These results suggest that C. olitorius leaves ameliorate PCOS symptoms in the studied biochemical and histological parameters.

کلیدواژه ها:

Hormonal imbalances ، Hyperandrogenism ، Exfoliative vaginal cytology ، polycystic ovarian syndrome (PCOS) ، Female fertility