Peganum harmala extract delayed the lethal effect of viper snake Echis carinatus venom in mice

This study evaluated possible antagonistic effects of Peganum harmala against the lethal effect of E. carinatus venom. Seventy-two albino mice were divided into ۱۲ equal groups in ۶ experimental protocols. In protocol I, Group A (control) treated with venom. They died after ۸۰ min averagely. In protocol II, Groups B۱ and B۲ were treated with ۱۵ and ۳۰ mg/kg of P. harmala, respectively and ۱۰ mg/kg venom simultaneously. Their survival time increased to ۲۳۲ and ۲۱۰ min respectively. In protocol III, groups C۱ and C۲ were treated with ۱۰ mg/kg venom and after ۱۵ min received P. harmala at ۱۵ and ۳۰ mg/kg respectively. Their time to death was increased to ۲۴۶ and ۲۲۰ min respectively. In protocol IV, groups D۱ and D۲, treated with pre-incubated of venom with P. harmala (۳۰ min) at the previous doses. Their survival time increased to ۲۱۱ and ۱۹۵ min respectively. In protocol V groups E۱ and E۲ received only P. harmala extract (IP) at ۱۵ and ۳۰ mg/kg respectively. In protocol VI, groups F۱, F۲, and F۳ received only P. harmala extract (orally) at doses of ۱۵, ۳۰, and ۶۰ mg/kg respectively, they remained alive. Peganum harmala significantly (p < ۰.۰۵) increased animal survival time thus, has an antagonistic effect against lethality effect of Echis carinatus venom, make it a possible antivenom candidate for further investigation.

کلیدواژه ها:

Snakebite ، Echis Carinatus ، venom ، Peganum Harmala ، antagonist ، Mice