Histopathological and Biochemical Effects of Natural Nano-Zeolite in Chronic Non-Bacterial Prostatitis of Rat Model

Chronic non-bacterial prostatitis (CNP) is a common condition characterized by chronic pelvic pain, inflammation, and oxidative stress (OS), negatively impacting the quality of life and fertility in men. In recent years, the mineral zeolite has attracted increasing attention in both human and veterinary medicine. Due to its ability to remove toxins and harmful metal cations, as well as its antioxidant properties, zeolite is used as an immune-enhancing agent for both animals and humans. Zeolite exhibits anti-inflammatory activity by reducing the expression of inflammatory cytokines. Given the limitations of current treatments, this study investigates for the first time the anti-inflammatory and antioxidant effects of zeolite (ZEO) in a carrageenan-induced CNP model in rats. ۳۰ male Sprague-Dawley rats were divided into ۵ groups: Sham, CNP, Cernilton, ZEO-IP, and ZEO-Oral. Prostatitis was induced using carrageenan, and treatments were administered for three weeks. Serum levels of PSA, IL-۶, and IL-۱۷ were measured using the ELISA method. Histological evaluations were also conducted to assess inflammation, hyperplasia, and hyperemia in prostate tissue. The highest levels of PSA, IL-۶, and IL-۱۷ were observed in the CNP group, while the groups treated with ZEO exhibited a reduction in these markers. However, this reduction was not statistically significant. Histopathological examinations revealed that severe inflammation in the CNP group was reduced in the ZEO-treated groups, particularly in the ZEO-Oral group. ZEO demonstrated potential therapeutic effects in CNP and improved the prostate tissue structure by lowering inflammation and OS. It is important to note that in the histopathological assessment, the alleviation of inflammatory conditions, hyperplasia, and hyperemia in the ZEO-treated groups was more pronounced compared to parameters such as PSA, IL-۶, and IL-۱۷. Therefore, although these results were not statistically significant, they suggest that ZEO may be used as a complementary treatment for CNP; however, further research is needed to confirm its clinical effects.

کلیدواژه ها:

Non-bacterial prostatitis ، Hyperplasia ، Oxidative Stress ، Zeolite