Assessment of the Frequency of qnrS and qnrA Genes in Clinical Isolates of Klebsiella pneumoniae and Their Relationship with Antibiotic Resistance Patterns and Iron Oxide Nanoparticles

Background: Klebsiella pneumoniae is a gram-negative, short, facultative anaerobic bacillus that commonly grows in laboratory culture environments, including blood agar, EMB, Mueller-Hinton agar, and nutrient agar. As an opportunistic pathogen, it poses significant treatment challenges due to increasing antibiotic resistance. Objectives: The purpose of this study is to determine the relationship between antibiotic resistance and the frequency of qnrS and qnrA genes in clinical isolates of Klebsiella pneumonia. Methods: In this study, ۱۰۰ patients referred to Sina Hospital in Tabriz due to urinary tract infections (UTIs) were examined. Common biochemical tests confirmed the presence of Klebsiella in ۲۰ samples. The antibiotic sensitivity pattern of the isolates was determined using the disc diffusion method, and the results of the antibiogram test were analyzed. The resistance of the samples to five different types of antibiotics was assessed. Results: In this study, the highest antibiotic resistance was observed against nalidixic acid. The presence of the qnrS gene was confirmed in five isolates of Klebsiella pneumoniae, while the qnrA gene was absent in all isolates. The sensitivity of the Klebsiella pneumoniae strain to iron oxide nanoparticles was evaluated using the well method at a concentration of ۰.۰۸ g in ۱۰۰ ml of water. One sample in a volume of ۱۰۰ ml and two samples in a volume of ۲۰۰ ml formed halos with diameters of ۱۱ mm, ۱۴ mm, and ۱۲ mm, respectively. Conclusion: The findings of the present study showed that the frequency of qnrS genes was high in fluoroquinolone-resistant samples of Klebsiella pneumoniae, and this factor could lead to progressive antimicrobial resistance in different hospital departments.

کلیدواژه ها:

Klebsiella pneumoniae ، Antibiotic Resistance ، qnrA Gene ، qnrS Gene ، Iron Oxide Nanoparticles