Vitamin D's Impact on Serum Exosome IL-۸ Gene Expression, Biochemical and Microscopic Study in PCOS Rats: An Experimental Study

Introduction: Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder affecting ۵-۱۵% of women of reproductive age, presenting symptoms such as elevated androgen levels and irregular ovulation. Recent research highlights the significance of inflammatory markers, particularly interleukin-۸ (IL-۸). Additionally, vitamin D deficiency may worsen inflammation, suggesting therapeutic benefits of supplementation. This research aimed to examine the impact of vitamin D therapy on the ovaries of female rats with induced PCOS using biochemical and microscopic methodologies. Methods: In experimental study, twenty-four pre-pubertal female Wistar rats (۴-۵ wk, ۹۰-۱۰۰ gr) were divided into four groups: control, laboratory control, PCOS, and treatment. The PCOS group received subcutaneous injections of ۶ mg/kg/day dehydroepiandrosterone (DHEA) in sesame oil and ethanol. The treatment group received the same DHEA dosage with ۱۲۰ ng/۱۰۰ g/week of ۱,۲۵ (OH)۲D۳. The laboratory control group was injected with sesame oil and ethanol, while the control group received only the vehicle. After ۲۸ days, blood samples and ovarian tissues were collected for analysis. Results: Elevated levels of FSH, LH, and testosterone were noted in the PCOS group compared to controls. The treatment group exhibited lower hormone levels than the PCOS group. Light microscopy revealed increased atretic and cystic follicles in the PCOS group, while vitamin D treatment significantly reduced these follicle types. IL-۸ levels were higher in the PCOS group, but vitamin D treatment significantly decreased them. Conclusion: The findings suggest that vitamin D treatment plays a therapeutic role in addressing androgen excess in a rat model of PCOS, benefiting hormonal and structural alterations associated with the condition.

کلیدواژه ها:

Polycystic ovary syndrome ، Vitamin D ، Interleukin-۸ ، Follicle stimulating hormone ، Luteinizing hormone