Serological and molecular evidence of respiratory viral mixed infection in sheep and goats

سال انتشار: 1404
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 23

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شناسه ملی سند علمی:

JR_VRFAN-16-9_001

تاریخ نمایه سازی: 30 شهریور 1404

چکیده مقاله:

Respiratory infections are considered within the major constraints of animal production; viruses are the major causing pathogens. This study aimed to elucidate the prevalence of parainfluenza virus-۳ (PIV-۳), bovine viral diarrhea virus, and respiratory syncytial virus (RSV) in sheep and goats and the existence of co-infections. A total of ۲۷۰ sheep and ۲۲۰ goat pneumonic lung tissues were collected from slaughterhouses in four different areas. Enzyme-linked immunosorbent assay was used to detect the antigen of the three viruses, fluorescent antibody technique and polymerase chain reaction confirmed enzyme-linked immuno-sorbent assay positive results. Prevalence detected for PIV-۳ was ۱۱.۱۰% in sheep and ۹.۵۰% in goats, pestivirus was ۱۰.۴۰% in sheep and ۷.۷۰% in goats, and RSV was ۱۷.۸۰% in sheep and ۵.۰۰% in goats. Detected co-infections were ۵.۶۰% for PIV-۳ and pestivirus in sheep and ۴.۰۰% in goats and pestivirus and RSV was observed only in goats (۱.۴۰%). Co-infection of the three viruses was detected in only one goat sample (۱.۰۰%). The existence of the three viruses in sheep and goats was confirmed. To our knowledge, this is the first report of the co-infections of PIV-۳, pestivirus, and RSV in sheep and goats in the studied areas.

نویسندگان

Yahia Ali

Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia

Intisar Saeed

Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia

Muaz Abdellatif

Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia

Alaa Mustafa

Department of Mathematic, College of Science and Arts, Northern Border University, Arar, Saudi Arabia

Hind Rikabi

Department of Pharmacy Practice, College of Pharmacy, Northern Border University, Arar, Saudi Arabia

Husham AlHassan Attaalfadeel

Department of Mathematic, College of Science, Northern Border University, Arar, Saudi Arabia