Temperature-induced physiological and phytochemical changes in Ziziphora clinopodioides during the growing season

Temperature has a profound effect on plant growth, development, and phytochemical production. The aim of this study was to evaluate the physiological and phytochemical responses of Ziziphora clinopodioides L. to decreasing temperatures throughout the growing season, focusing on nitrogen levels, chlorophyll content, and essential oil composition. By identifying these changes, we sought to determine the optimal growing conditions to improve the quality and industrial applications of this medicinal plant. The investigation was conducted through a field experiment in ۲۰۲۲ at the Faculty of Agriculture, Azarbaijan Shahid Madani University. The experiment followed a randomized complete block design. The results showed a significant relationship between decreasing ambient temperatures and the phytochemical components of the plant. As the temperature decreased, there was a corresponding reduction in the levels of chlorophyll, nitrogen, and essential oils. The results showed an inverse relationship between essential oil content and pulegone with decreasing air temperature. In contrast, menthone, menthol, and piperitone showed a positive correlation with cooler temperatures. Pulegone content decreased by ۸۱.۷۵% from the first sampling at ۲۲.۵°C to the sixth sampling at ۷°C, with the highest concentration observed at the highest temperature (۲۲.۵°C) and the lowest at the lowest temperature (-۱°C). Conversely, menthone and menthol concentrations were minimal at the highest temperature (۲۲.۵°C) during the first stage and peaked at the seventh stage when temperatures reached ۵.۵°C. These results provided valuable insights into the biochemical responses of Z. clinopodioides to temperature variation, enhancing our understanding of its essential oil quality and potential industrial applications.

کلیدواژه ها:

Essential oil ، Leaf temperature ، Pulegone ، Temperature stress ، Essential oil ، Leaf temperature ، Pulegone ، Temperature stress