Dual Nanoformulations of 𝑪𝒆𝒏𝒕𝒆𝒍𝒍𝒂 𝒂𝒔𝒊𝒂𝒕𝒊𝒄𝒂 and Rosmarinus Officinalis: Effects on IL-۱۰ Cytokine and Collagen I Biomarkers in Incisional Wounds

Improving postoperative incisional wound healing remains a challenge. Centella asiatica and Rosmarinus officinalis possess anti-inflammatory and collagen-promoting properties, but poor skin permeability limits their therapeutic effectiveness. This study evaluated the effects of a dual nanoparticle gel combining transfersomal C. asiatica extract and rosemary oil nanoemulsion on wound closure, IL-۱۰ expression, and collagen type I deposition. Twenty-five female Wistar rats (n=۵/group) received standardized ۳ cm midline abdominal incisions. The groups included no treatment, standard gel (۳% C. asiatica + ۱% rosemary oil), and transfersomal gels containing ۰.۱۵, ۰.۳۰, or ۰.۶۰% C. asiatica plus ۰.۱% rosemary nanoemulsion. The treatments were applied topically once daily for ۱۴ days. Wound closure was assessed using digital photography and ImageJ software. IL-۱۰ and collagen I expression were quantified by immunohistochemistry at ۱,۰۰۰× magnification. Early wound closure (days ۰–۷) showed no significant difference (p>۰.۰۵). By day ۱۰, the ۰.۳۰% and ۰.۶۰% transfersome groups achieved significantly greater closure (۶۳% and ۶۷%, respectively) than the controls (۳۰–۳۸%, p<۰.۰۵). On day ۱۴, they reached ۷۹% and ۸۲%, respectively, compared to ۵۹-۶۱% in the controls. IL-۱۰ expression peaked in the ۰.۶۰% group (۱۱.۸ AU), while collagen I deposition was also highest (۱۹.۸ AU), both significantly surpassing the other groups (p<۰.۰۵). Transfersomal C. asiatica and rosemary nanoemulsion gels enhanced transdermal delivery, increased anti-inflammatory signaling, and promoted extracellular matrix remodeling, significantly accelerating wound healing. This formulation shows promise as topical therapy for surgical wounds.

کلیدواژه ها:

𝐶𝑒𝑛𝑡𝑒𝑙𝑙𝑎 𝑎𝑠𝑖𝑎𝑡𝑖𝑐𝑎 ، 𝑅𝑜𝑠𝑚𝑎𝑟𝑖𝑛𝑢𝑠 𝑜𝑓𝑓𝑖𝑐𝑖𝑛𝑎𝑙𝑖𝑠 ، wound healing ، IL-۱۰ ، Collagen Type I ، Nanoparticles