Bioactivity of Nano 𝑪𝒖𝒓𝒄𝒖𝒎𝒂 𝒍𝒐𝒏𝒈𝒂 Encapsulated in Carboxymethyl Chitosan

Nanoencapsulation of C. longa extract with carboxymethyl chitosan (CMCs) was formulated to prevent its bioactive deterioration in anti-dengue and anti-cancer activities. The particle size of C۱-NPs was measured at ۴۶۲.۵ ± ۱۰.۸۷ nm with a PDI value of ۰.۲۸ ± ۰.۰۵ which indicates homogeneous particle size distribution. C۱-NPs were observed to be stable at ۳۰-۱۰۰ °C but less stable at high pH and salt concentrations. C۱-NPs had a %LE of ۱۹.۷۷ ± ۷.۴۶% and %LC of ۳۹.۵۴ ± ۱۴.۹۱%. The release of C۱-NPs at pH ۲, was observed at ۲.۸۹ ± ۰.۳۳%; at pH ۷, it was ۸.۵۰ ± ۰.۹۸%; and at pH ۸.۵, it was ۵.۸۹ ± ۰.۴۰%. The best release was observed at pH ۷. Anticancer activity against the MCF-۷ cell line showed that C۱-NPs had better inhibitory activity compared to C. longa. C۱-NPs had an EC۵۰ value of ۴.۷۵ ± ۰.۴۲ µg/mL, while C. longa had ۳۳.۰۳ ± ۷.۲۰ µg/mL. C. longa extract and C۱-NPs exhibited anti-dengue activity with EC۵۰ values of ۵۴.۰۹ ± ۱۱.۸۴ and ۹.۰۲ ± ۰.۰۰ µg/mL, respectively. Based on the selectivity index (SI), C۱-NPs are more selective than the C. longa extract. Proven to improve bioavailability and controlled release, C. longa extract nanoencapsulation enables precise targeting of bioactive compounds to enhance bioactivity.

کلیدواژه ها:

Nanocurcumin ، Antivirus ، Anticancer ، DENV ، breast cancer ، CMC