Characterization, Antioxidant Potential, and 𝐼𝑛 𝑆𝑖𝑙𝑖𝑐𝑜 Study of 𝑅𝑢𝑒𝑙𝑙𝑖𝑎 𝑇𝑢𝑏𝑒𝑟𝑜𝑠𝑎 Hydroethanol Extract of Leaf

This study was conducted to evaluate the phytochemical profile, alpha-amylase inhibitory potential, and in vitro antioxidant activity of the hydro-ethanolic [۷۰:۳۰] extract from Ruellia tuberosa leaves, along with molecular docking with catalase (۷VD۹), PPARγ (۲P۴Y), and AChE (۴PQE) enzymes. Fresh leaves were collected, purified, and air-dried prior to extraction with hydroethanol to obtain crude extracts that were rich in secondary metabolites. Qualitative phytochemical screening has revealed the presence of flavonoids, phenolic acids, tannins, and other beneficial compounds. LC-MS analysis provided a comprehensive profile of these compounds, identifying numerous peaks corresponding to established anti-diabetic medications and antioxidants. The antioxidant capacity of the extract was evaluated by DPPH, ABTS, nitric oxide radical scavenging, and hydroxyl free radical assays with IC۵۰ values at the concentration of ۷۰, ۳۰۵, ۳۵۰, and ۱۵۵ μg/ml, respectively, demonstrating a substantial dose-dependent capacity for free radical scavenging. The hydroethanol extract demonstrated significant alpha amylase inhibitory activity (IC۵۰ ۴۴±۰.۱۸ μg/mL), indicating its potential to regulate glucose metabolism and aid in the management of postprandial hyperglycemia. The interactions among the identified phytochemicals may explain the observed anti-diabetic and antioxidant effects. These findings validate the traditional application of R. tuberosa in addressing metabolic diseases and oxidative stress, while highlighting the potential of the plant as a source of natural therapeutic agents. The molecular docking study of the RTL extract revealed the strong interactions of its key phytochemicals, catechin, amyrin, and kaempferol, with the target proteins catalase, PPARγ, and AChE. These compounds exhibited significant binding affinities, suggesting potential therapeutic activities. These in silico findings support the bioactivity observed in in in-vitro assays and provide a basis for further pharmacological research. Additional studies, including isolation of specific compounds and in vivo testing, are necessary to elucidate the mechanisms of action and inform the development of novel pharmacological and nutraceutical applications.

کلیدواژه ها:

Ruellia Tuberosa ، Leaf Extraction ، α-amylase activity ، In vitro antioxidant study