Comparison of the effects of platelet-rich plasma and nanocurcumin on the sperm quality parameters in frozen-thawed semen of men with asthenoteratozoospermia: A lab trial study

Background: Cryopreservation of sperm can adversely affect sperm quality, particularly in individuals with asthenoteratozoospermia. Various substances are added to the cryopreservation medium to prevent cellular damage. Objective: This study aimed to evaluate the effects of platelet-rich plasma (PRP) and nanocurcumin (nCur) on sperm parameters, DNA fragmentation, and oxidative stress levels in cryopreserved semen. Materials and Methods: In this lab trial study, semen samples of ۲۰ men with asthenoteratozoospermia who referred to Rastak and Ghavamzadeh Infertility Centers, Arak, Iran from June-August ۲۰۲۴ were collected. Each sample was divided into ۵ groups: control (no treatment), PRP۵۰, PRP۱۰۰, nCur۱۰, and nCur۲۰. The control samples underwent freezing without additives. Post-thawing assessments included sperm motility, viability, DNA fragmentation index, and malondialdehyde levels to evaluate oxidative stress. Results: Treatment with PRP۵۰ (p < ۰.۰۰۱) and nCur۲۰ (p = ۰.۰۰۱) significantly increased sperm motility compared to the control group. All treatment groups showed a significant increase in viable sperm following freezing (p < ۰.۰۰۱), with PRP۵۰ demonstrating the most pronounced effects (p < ۰.۰۰۱). While no improvements in sperm morphology were observed after freezing, PRP and nCur significantly reduced DNA fragmentation index after freezing (p < ۰.۰۰۱), with PRP۵۰ being the most effective (p < ۰.۰۰۱). Additionally, both PRP and nCur significantly decreased malondialdehyde levels compared to the control group (p < ۰.۰۰۱). Conclusion: PRP and nCur effectively improve sperm parameters in cryopreserved semen from individuals with asthenoteratozoospermia, particularly emphasizing the superior efficacy of PRP at a concentration of ۵۰ in improving sperm quality. These results support the potential use of these agents as additives in sperm cryopreservation protocols to improve reproductive outcomes.

کلیدواژه ها:

Cryopreservation ، Spermatozoa ، Asthenoteratozoospermia ، Platelet-rich plasma ، Nanocurcumin. ، انجماد ، اسپرماتوزوآ ، آستنوتراتوزواسپرمی ، پلاسمای غنی شده از پلاکت ، نانوکورکومین.