Studying Repetitive Genetic Units Using Electrophoresis and Sequencing Methods for the Identification of Burkholderia mallei

Burkholderia mallei, the causative agent of Glanders, are a zoonotic infectious disease that affects domestic animals, particularly horses, donkeys, and mules, and can also infect humans. Due to its high transmissibility and cross-species spread, Glanders is recognized as a significant threat to public health and biosafety. Controlling and eradicating this disease require precise and reliable diagnostic methods to detect and confirm the presence of the bacterium in clinical and environmental samples. This study focused on evaluating advanced molecular techniques, specifically gel electrophoresis and DNA sequencing, for accurate identification and differentiation of B. mallei strains. The primary aim was to assess the effectiveness of these techniques in diagnosing B. mallei and to provide a framework for epidemiological studies and disease control in Iran. The results demonstrated that electrophoresis and sequencing are powerful tools for accurate identification and strain differentiation of B. mallei. By providing detailed genetic information, these techniques can play a critical role in epidemiological research, tracking infection sources, and monitoring disease spread. Given the importance of Glanders in Iran and its potential zoonotic transmission, developing and implementing these molecular methods within the country’s veterinary and healthcare systems is essential. Based on this, the following recommendations are proposed: ۱- Focus on Iranian clinical isolates ۲- Increase the number of genetic loci studied ۳- Develop standardized protocols International collaborations and data exchange, through implementing these recommendations, can lead to deeper insights into the epidemiology of Ganders in Iran and enhance control and eradication programs with stronger scientific and technical backing. This represents a significant step toward protecting animal and human health and reducing economic losses caused by this disease.

کلیدواژه ها:

Burkholderia mallei ، Glanders ، gel electrophoresis ، DNA sequencing ، PCR ، genotyping ، molecular markers ، epidemiology ، disease control