Diagnosis of COVID -۱۹ by PCR method from the nasopharyngeal samples of symptomatic domestic cats and dogs

سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 202

فایل این مقاله در 5 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_JCBIOR-5-2_004

تاریخ نمایه سازی: 3 شهریور 1404

چکیده مقاله:

The coronavirus disease ۲۰۱۹ (COVID-۱۹) is caused by the acute respiratory syndrome of the coronavirus-۲ (SARS-CoV-۲). Due to its severity and spread, the World Health Organization considered it an epidemic. Coronavirus can infect several species of animals and can spread to people and then spread between them. This study aimed to isolate COVID-۱۹ from the nasopharynx of cats and dogs. This cross-sectional study was conducted over ۳ months, spring ۲۰۲۲. Saliva and nasopharynx swab samples were taken from ۳۶ domestic dogs (N=۳۰) and cats (N=۶) that were symptomatic (with respiratory and/or digestive symptoms) in the clinic of the Faculty of Veterinary Medicine of Tehran University. Also, a questionnaire specific to the owners of these animals was considered, including questions such as age, sex, history of most recently COVID-۱۹, and clinical symptoms. COVID-۱۹ was detected by the real-time polymerase chain reaction (Real-Time PCR) method. In this study ۷ dog parents (۲۳.۳%) were recently infected with COVID-۱۹, of which ۴ had contact with their pets. Also, one cat parent had been recently infected and had contact with other pets during his illness. No COVID-۱۹ was isolated from the symptomatic pets, but some clinical symptoms were reported in them; the most common symptoms seen in dogs were digestive and then respiratory ones. In cats, the most involved systems were respiratory and then Gastrointestinal. All saliva and nasopharynx samples were negative. It will require more samples of pets of more individuals with confirmed SARS-CoV-۲ infection in future studies. Nevertheless, avoiding close contact should be considered as long as they remain ill, and using face masks and basic health measures should be observed.The coronavirus disease ۲۰۱۹ (COVID-۱۹) is caused by the acute respiratory syndrome of the coronavirus-۲ (SARS-CoV-۲). Due to its severity and spread, the World Health Organization considered it an epidemic. Coronavirus can infect several species of animals and can spread to people and then spread between them. This study aimed to isolate COVID-۱۹ from the nasopharynx of cats and dogs. This cross-sectional study was conducted over ۳ months, spring ۲۰۲۲. Saliva and nasopharynx swab samples were taken from ۳۶ domestic dogs (N=۳۰) and cats (N=۶) that were symptomatic (with respiratory and/or digestive symptoms) in the clinic of the Faculty of Veterinary Medicine of Tehran University. Also, a questionnaire specific to the owners of these animals was considered, including questions such as age, sex, history of most recently COVID-۱۹, and clinical symptoms. COVID-۱۹ was detected by the real-time polymerase chain reaction (Real-Time PCR) method. In this study ۷ dog parents (۲۳.۳%) were recently infected with COVID-۱۹, of which ۴ had contact with their pets. Also, one cat parent had been recently infected and had contact with other pets during his illness. No COVID-۱۹ was isolated from the symptomatic pets, but some clinical symptoms were reported in them; the most common symptoms seen in dogs were digestive and then respiratory ones. In cats, the most involved systems were respiratory and then Gastrointestinal. All saliva and nasopharynx samples were negative. It will require more samples of pets of more individuals with confirmed SARS-CoV-۲ infection in future studies. Nevertheless, avoiding close contact should be considered as long as they remain ill, and using face masks and basic health measures should be observed.

کلیدواژه ها:

نویسندگان

Seyedeh Mahsan Hoseini Alfatemi

Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abdolali Malmasi

Department of Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Leila Azimi

Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Noushin Marhamati

Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Hannan Khodaei

Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Ali Molavi Ardekani

Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran

Barsam Saberi

Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Alborz, Iran

Amin Bagheri

Department of Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Hirad Rokni

School of Dentistry, Islamic Azad University of Medical Sciences, Tehran, Iran

Seyed Hossein Ardehali

Department of Anesthesiology & Critical Care, Faculty of Medicine, Shohadaye-Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Bienzle D, Rousseau J, Marom D, MacNicol J, Jacobson L, ...
  • Santaniello A, Perruolo G, Cristiano S, Agognon AL, Cabaro S, ...
  • Csiszar A, Jakab F, Valencak TG, Lanszki Z, Toth GE, ...
  • Jairak W, Charoenkul K, Chamsai E, Udom K, Chaiyawong S, ...
  • Goumenou M, Spandidos DA, Tsatsakis A. [Editorial] Possibility of transmission ...
  • Gaudreault NN, Carossino M, Morozov I, Trujillo JD, Meekins DA, ...
  • Gaudreault NN, Trujillo JD, Carossino M, Meekins DA, Morozov I, ...
  • Kumar D, Bayry J, Hegde NR. COVID-19: A Veterinary and ...
  • Shi J, Wen Z, Zhong G, Yang H, Wang C, ...
  • Stout AE, Andre NM, Jaimes JA, Millet JK, Whittaker GR. ...
  • Sit THC, Brackman CJ, Ip SM, Tam KWS, Law PYT, ...
  • Subedi S, Koirala S, Chai L. COVID-19 in Farm Animals: ...
  • Barbu MG, Thompson DC, Suciu N, Voinea SC, Cretoiu D, ...
  • Temmam S, Barbarino A, Maso D, Behillil S, Enouf V, ...
  • Ruiz-Arrondo I, Portillo A, Palomar AM, Santibanez S, Santibanez P, ...
  • Sailleau C, Dumarest M, Vanhomwegen J, Delaplace M, Caro V, ...
  • Ferasin L, Fritz M, Ferasin H, Becquart P, Corbet S, ...
  • Decaro N, Balboni A, Bertolotti L, Martino PA, Mazzei M, ...
  • نمایش کامل مراجع