Inhibition of mast cell activation via MRGPRX۲ receptor by Curcuma mangga and Sonchus arvensis water suspensions: An in vitro study

Objective: Traditional medicine is often used to relief pain, but its use is frequently not supported by appropriate scientific information. This study aims to investigate the histamine release suppression of Sonchus arvensis (SA) and Curcuma mangga (CM).Materials and Methods: Rat peritoneal mast cells (RPMCs) and Mas-related GPCR-X۲(MRGPRX۲)-transfected RBL-۲H۳ cells were activated by ۵۰ μg/ml of compound ۴۸/۸۰. Water suspension of SA or CM (۰.۱-۳۰ mg/ml) was used to inhibit cell activation by compound ۴۸/۸۰. The level of mast cell activation was determined by measuring histamine release concentration using HPLC-fluorometry.Results: At a concentration of ۱۰ mg/ml, CM resulted in ۲۲.۶۰±۵.۸۶% in a spontaneous histamine release from RPMCs. The net histamine release after compound ۴۸/۸۰ stimulation in RPMC was ۶۷.۱۹±۱.۳۱%. CM at ۳ mg/ml suppressed histamine release to ۸.۴۵±۲.۵۳%. In MRGPRX۲-transfected RBL-۲H۳ cells stimulated with compound ۴۸/۸۰, CM at concentrations of ۳ and ۱۰ mg/mL suppressed histamine release to ۲۲.۸۵±۰.۶۴% and ۴.۲۰±۱.۶۰%, respectively. SA at ۳۰ mg/ml induced a spontaneous histamine release of ۵۶.۷۶±۴.۰۳%, compared to ۵.۶۵±۲.۶۱% in the control group. The administration of ۳ mg/ml of SA to compound ۴۸/۸۰-stimulated RPMCs resulted in a net histamine release of ۶.۱۲±۰.۴۶%. In MRGPRX۲-transfected RBL-۲H۳ cells activated by compound ۴۸/۸۰, the net release was ۳۵.۱۱±۳.۱۰%. SA at ۱۰ mg/ml suppressed histamine release to ۴.۸۸±۱.۴۲%.Conclusion: SA and CM water suspension suppressed compound ۴۸/۸۰-induced histamine release.

کلیدواژه ها:

Traditional medicine Pain MRGPRX۲ ، transfected RBL ، ۲H۳ cells Rat peritoneal mast cells Histamine