Neurotoxicity of Isotretinoin in Mice: Behavioral and Tissue Neurological Function Assessment
محل انتشار: فصلنامه طب دامی ایران، دوره: 19، شماره: 4
سال انتشار: 1404
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 7
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شناسه ملی سند علمی:
JR_IJVM-19-4_008
تاریخ نمایه سازی: 27 مرداد 1404
چکیده مقاله:
Background: Isotretinoin is used to treat some skin disorders in dogs and cats by reducing the size and activity of their sebaceous glands, although it may have some neurobehavioral side effects.Objectives: To evaluate isotretinoin’s effects on the brain and neurotransmitters, as well as its impact on neurobehavior and motor activity. Methods: Fifteen mice were divided into three groups: the first group was a control group, the second group received ۱۲۵ mg/kg isotretinoin, and the third group received ۲۵۰ mg/kg orally. Results: The LD۵۰ for isotretinoin is ۴۸۴۱.۲ mg/kg. Neurobehavioral measurements of mice revealed significant effects on changes in open-field activity, time spent in dark areas, and negative geotaxis behaviors across different dosage levels of isotretinoin. Both doses of isotretinoin (۱۲۵ and ۲۵۰ mg/kg) significantly altered serotonin levels. Mice treated with ۱۲۵ mg/kg isotretinoin exhibited a decrease in serotonin levels compared to the control group. Both doses of isotretinoin resulted in significant changes in acetylcholine levels. Isotretinoin (۱۲۵ mg/kg) slightly increased in acetylcholine levels. The data indicated a significant increase in catechol-O-methyltransferase (COMT) enzyme levels. A histopathological study of the brain revealed that ۱۲۵ mg/kg isotritinoin induced mild vacuolization, blood vessel congestion, and mild perivascular edema. A high dose (۲۵۰ mg/kg) resulted in vacuolization, gliosis, blood vessel congestion, hemorrhage and satellitosis. Conclusion: High oral doses of isotretinoin influence animal neurobehavioral behavior due to its effect on brain tissue, as evidenced by its effects on serotonin, acetylcholine and the COMT enzyme.
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نویسندگان
Yamama Alabdaly
Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
Sahar Abdul Hameed
Department of Anesthesia, Medical Technical Institute, Northern Technical University, Mosul, Iraq.
Nada Ibrahim
Department of Anesthesia, Medical Technical Institute, Northern Technical University, Mosul, Iraq.