Exploring the Therapeutic Potential of Iphiona aucheri: Modulation of Nitric Oxide Levels in RAW ۲۶۴.۷ Macrophages and Cytotoxic Effects on MCF-۷ Human Breast Cancer and HT۱۰۸۰ Human Fibrosarcoma Cells

The objective of this study was to examine phytochemical compounds and the cellular effects of the essential oil of Iphiona aucheri (Boiss.) Anderb. The essential oil of the dried aerial parts of the plant with the recovery rate of ۰.۱% (v/w) was extracted through hydrodistillation by a Clavenger apparatus, and the chemical compounds were identified based on gas chromatography-mass spectrometry. The anti-inflammatory and cytotoxic effects of the essential oil were examined. The Griess assay was used to test the anti-inflammatory activity on the RAW ۲۶۴.۷ macrophage cell line. The MTT assay was used to test the cell viability on the RAW ۲۶۴.۷ macrophage, the HT۱۰۸۰ fibrosarcoma, and the MCF-۷ cell lines. The essential oil of this plant consists of sesquiterpenes and monoterpenes, with more than ۶۰% of it made up of compounds such as limonene, α-farnesene, and trans-caryophyllene. The essential oil demonstrated its anti-inflammatory effect on macrophage cells (RAW ۲۶۴.۷) by reducing nitric oxide production at concentrations below ۱۰۰ µg/mL.  Increasing the cell viability percentage of macrophage cells along with reducing the NO release rate at a concentration of ۶.۲۵ μg/mL suggested promising anti-inflammatory effects. The essential oil was cytotoxic to the human fibrosarcoma (HT۱۰۸۰) with an IC۵۰ of ۴۷.۹۴±۱ µg/mL.

کلیدواژه ها:

Iphiona aucheri ، Anti-inflammatory ، nitric oxide ، Cell viability