A Comparative Analysis of the Antibacterial Effects of Green-Synthesized Silver Nanoparticles with Chemically Synthesized Silver Nanoparticles

Background: In recent years, the antimicrobial properties of nanoparticles have received significant attention due to increasing bacterial resistance to conventional antibiotics. In this study the antibacterial effects of green-synthesized silver nanoparticles with chemically synthesized silver nanoparticles have been comprised. Methods: The physicochemical properties of the synthesized nanoparticles were evaluated through Ultraviolet-Visible (UV-Vis) spectrophotometry, Dynamic Light Scattering (DLS), Fourier Transform Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FESEM), and Energy Dispersive X-ray Analysis (EDAX) and antimicrobial activity by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. Results: DLS analysis revealed average sizes of ۷۲.۸ nm and ۱۲۴.۸ nm for green-synthesized and chemically synthesized AgNPs, respectively. FESEM images showed spherical shapes, while EDAX confirmed the presence of silver, carbon, and oxygen in the nanoparticles. Antimicrobial testing (MIC and MBC) showed that green-synthesized AgNPs exhibited stronger antibacterial activity than chemically synthesized ones. For Escherichia coli, the MIC and MBC for green-synthesized AgNPs were ۰.۰۲۸ mg/mL and ۰.۰۵۶ mg/mL, respectively, compared to ۰.۱۲۵ mg/mL and ۰.۲۵ mg/mL for chemically synthesized AgNPs. Similarly, for Staphylococcus aureus, the MIC and MBC values for green-synthesized nanoparticles were ۰.۲۲۶ mg/mL, while chemically synthesized nanoparticles had MIC and MBC values of ۰.۱۲۵ mg/mL and ۰.۲۵ mg/mL. Conclusion: These results demonstrated a higher antimicrobial potential of plant-mediated silver nanoparticle synthesis over chemical methods, especially against Gram-negative bacteria.

کلیدواژه ها:

Silver nanoparticles ، Green synthesis ، Agrimonia eupatoria L ، Antibacterial activity