Extraction, Phytochemical Screening, Green Synthesis of Silver Nanoparticles (Ag-NPs), and Antioxidant Potential of 𝐴𝑝ℎ𝑎𝑛𝑎𝑚𝑖𝑥𝑖𝑠 𝑝𝑜𝑙𝑦𝑠𝑡𝑎𝑐ℎ𝑦𝑎

This study reports the green synthesis of silver nanoparticles (Ag-NPs) using an aqueous leaf extract of Aphanamixis polystachya, a plant known for its medicinal properties. The biosynthesized Ag-NPs were characterized using UV-Vis spectroscopy, FTIR, SEM, XRD, DLS, and zeta potential analysis. The UV-Vis spectrum displayed a surface plasmon resonance peak at ۴۲۵ nm, and particle size analysis showed an optimized average diameter of ۳۹.۷ nm. Zeta potential was recorded at -۹.۲۹ mV, confirming colloidal stability, while entrapment efficiency reached ۸۲.۲%. Functional groups such as O–H, C=O, and C–N identified by FTIR analysis facilitated nanoparticle formation and stabilization. SEM revealed spherical morphology and XRD confirmed a crystalline face-centered cubic structure with peaks at ۳۸.۱°, ۴۴.۳°, ۶۴.۴°, and ۷۷.۴°. In vitro antioxidant evaluation using DPPH assay demonstrated superior activity of Ag-NPs (IC۵۰ = ۰.۶۸ ± ۰.۰۶ mg/mL) over the crude leaf extract (IC۵۰ = ۱.۵۷ ± ۰.۰۷ mg/mL). Additionally, the Ag-NPs exhibited a drug release of ۹۸.۲۷ ± ۲.۷۶% over ۱۲ hours and remained physically stable over three months. These findings imply that the leaf extract of A. polystachya functions as a sustainable, eco-friendly, and efficient reducing and capping agent for the production of stable Ag-NPs, with encouraging prospects for additional biological and medicinal uses.

کلیدواژه ها:

Plant extracts ، Ag-NPs ، Spectroscopy ، A. polystachya ، Leaf extract