A nanoparticle loaded nasal sol-gel system of midazolam hydrochloride for the management of intermittent seizures

Objective(s): Midazolam hydrochloride is a short-acting hypnotic sedative with anticonvulsant properties. Considering the poor bioavailability, rapid elimination, and inconvenience of administering oral medication in epileptic conditions, this study proposed the use of a nanoparticulate in situ nasal gel system for midazolam to achieve an optimum therapeutic effect.Materials and Methods: Drug-loaded PLGA nanoparticles were prepared by an emulsion-solvent evaporation method utilizing a Box–Behnken design concentrated on particle size, zeta potential, and drug entrapment efficiency as responses. The optimized nanoparticles were incorporated into a ۰.۲% w/v gellan gum solution. The nanoparticle-loaded gel was studied for ex vivo drug permeation through excised sheep nasal mucosa. The anticonvulsive activity of the gel was compared with that of the marketed midazolam nasal spray (۰.۵ mg/۰.۱ ml).Results: Particle size (۲۰۰ nm to ۳۱۰ nm), zeta potential (-۲۵mV to -۳۱mV), and entrapment efficiency (۸۱.۲۳±۰.۲۱ to ۹۳.۳۲±۰.۲۸%) were observed in the formulations. The drug release from the formulations over ۲۴ h was found to be ۷۵.۳۲±۰.۰۲۸to ۸۴.۶۳±۰.۰۶۱%. Particle size and zeta potential were best fitted to the quadratic model, and an entrapment efficiency linear model was suggested. The ex vivo permeation studies of the optimized nanoparticle-incorporated gel formulation exhibited a fourfold increase in flux and permeability coefficient in comparison to the pure drug-incorporated gel. The anticonvulsive effect in pentylenetetrazol (PTZ)-induced rat model epilepsy showed a similar anticonvulsive profile to that of the marketed nasal spray.Conclusion: Drug-loaded nanoparticles incorporated in situ gel would be a promising, biocompatible, and non-invasive approach to achieve the required therapeutic efficacy by sustained and direct action on brain cells

کلیدواژه ها:

Nasal ، Gellan gum ، Midazolam ، Nanoparticles ، PLGA