Expression of ADAM۱۰ and CD۵۸ in Acute and Chronic Lymphocytic Leukemia: Influence of Disease Stage and Chemotherapy

Background & Objective: CD۵۸ and ADAM۱۰ have been implicated in leukemia progression and chemoresistance; however, their specific roles in acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL), particularly under chemotherapeutic pressure, remain insufficiently characterized. This study aimed to assess the expression of CD۵۸, an immune adhesion molecule, and ADAM۱۰, a metalloproteinase, in ALL and CLL patients undergoing chemotherapy, and to explore their potential involvement in immune evasion, niche-mediated survival, and chemoresistance mechanisms.Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from ۵۰ patients with ALL, ۵۰ with CLL, and ۳۰ healthy controls. Expression levels of CD۵۸ and ADAM۱۰ were analyzed by quantitative reverse transcription PCR (qRT-PCR) and flow cytometry. Chemotherapy regimens included vincristine (VCR), methotrexate (MTX), and doxorubicin (DOXO).Results: ADAM۱۰ mRNA expression was significantly upregulated in ALL patients treated with VCR+MTX (p<۰.۰۰۰۱) and DOXO (p=۰.۰۰۱), with corresponding protein overexpression observed in both ALL (p<۰.۰۰۰۱) and untreated CLL patients (p<۰.۰۰۰۱). A significant difference in ADAM۱۰ levels was noted between ALL and CLL cohorts (p=۰.۰۰۱). CD۵۸ mRNA and protein expression were markedly increased in ALL patients receiving VCR+MTX (p<۰.۰۰۰۱), whereas untreated CLL patients exhibited no significant alterations.Conclusion: CD۵۸ and ADAM۱۰ are differentially regulated in ALL under chemotherapy, supporting their roles in immune evasion and microenvironmental survival. The constitutive overexpression of ADAM۱۰ in CLL suggests its involvement in chronic leukemic pathogenesis. These findings highlight CD۵۸ and ADAM۱۰ as potential therapeutic targets for overcoming chemoresistance in lymphoid malignancies.

کلیدواژه ها:

CD۵۸ ، ADAM۱۰ ، Acute lymphoblastic leukemia ، Chronic lymphocytic leukemia ، doxorubicin ، vincristine ، Methotrexate