Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep Feces

سال انتشار: 1404
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 34

فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_IJVST-17-1_008

تاریخ نمایه سازی: 25 تیر 1404

چکیده مقاله:

Actinomycetes are a vital group of Gram-positive bacteria known for producing a wide range of bioactive secondary metabolites, including important antibiotics. These microorganisms play an essential role in the degradation of organic matter and nutrient cycling, contributing significantly to soil health and fertility. Their capacity to synthesize diverse compounds and the presence of key biosynthetic pathways involving polyketide synthases and non-ribosomal peptide synthetases highlights their potential in antibiotic discovery, particularly against antibiotic-resistant pathogens. This study aimed to isolate and characterize Actinomycetes from fresh sheep feces collected in Ilam Province, Iran, focusing on their antibacterial activity and biosynthetic potential. A total of ۸۶ actinomycete isolates were obtained from fecal samples collected from sheep in ۲۰۲۱. Morphological characterization confirmed all isolates as Gram-positive and filamentous. Molecular identification through PCR amplification of the ۱۶S rRNA gene yielded a product of approximately ۶۴۰ base pairs for all isolates. Antibacterial screening revealed that ۱۷ isolates exhibited activity against various pathogens, with the highest efficacy observed against Bacillus cereus (۶۲.۱%). Molecular analysis also indicated the presence of biosynthetic gene clusters, with ۳۱ isolates (۳۶.۰۵%) bearing non-ribosomal peptide synthetase (NRPS) gene, ۱۵ isolates (۱۷.۴۴%) containing polyketide synthase I (PKS-I), and ۱۶ isolates (۱۸.۶%) with polyketide synthase II (PKS-II) genes. This study highlights the significant antibacterial properties and biosynthetic capabilities of actinomycetes from sheep feces, suggesting their potential use in therapeutic, agriculture, and biotechnological applications.

نویسندگان

Talieh Mohammadi

Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran

Fazel Pourahmad

Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran

Khalil Saleki

Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Chaudhary HS, Soni B, Shrivastava AR, Shrivastava S. Diversity and ...
  • Lacey HJ, Rutledge PJ. Recently discovered secondary metabolites from Streptomyces ...
  • Singh V, Haque S, Singh H, Verma J, Vibha K, ...
  • Selim MSM, Abdelhamid SA, Mohamed SS. Secondary metabolites and biodiversity ...
  • Trenozhnikova LP, Baimakhanova GB, Baimakhanova BB, Balgimbayeva AS, Daugaliyeva ST, ...
  • Kaale SE, Machangu RS, Lyimo TJ. Molecular characterization and phylogenetic ...
  • Shrestha B, Nath DK, Maharjan A, Poudel A, Pradhan RN, ...
  • Behie SW, Bonet B, Zacharia VM, McClung DJ, Traxler MF. ...
  • Masand M, Jose PA, Menghani E, Jebakumar SR. Continuing hunt ...
  • Hayashida S, Nanri N, Teramoto Y, Nishimoto T, Ohta K, ...
  • Dentzien-Dias P, Poinar G, Francischini H. A new actinomycete from ...
  • Lee HW, Ahn JH, Kim M, Weon HY, Song J, ...
  • Wang X, Zhang Z, Wang X, Bao Q, Wang R, ...
  • Ahmad A, Sur S. Biodegradable solid waste management by microorganism: ...
  • Game BC, Deokar CD, Jadhav AC. Characterization of cellulolytic microorganisms ...
  • Sánchez ÓJ, Ospina DA, Montoya S. Compost supplementation with nutrients ...
  • Forster SM. The role of microorganisms in aggregate formation and ...
  • Abdelrahman OY, Yagi S, El Siddig M, El Hussein A, ...
  • Hwang S, Lee N, Cho S, Palsson B, Cho BK. ...
  • Wang H, Fewer DP, Holm L, Rouhiainen L, Sivonen K. ...
  • Quadri LE. Assembly of aryl‐capped siderophores by modular peptide synthetases ...
  • Lee LH, Zainal N, Azman AS, Eng SK, Goh BH, ...
  • Tatar D. Isolation, phylogenetic analysis and antimicrobial activity of halophilic ...
  • Okada BK, Seyedsayamdost MR. Antibiotic dialogues: Induction of silent biosynthetic ...
  • Lima SMA, Pereira PS, da Silva BIM, Ribeiro NE, de ...
  • Shikuku BO, Kiruki S, Kuria E, Mutembei M, Ogolla FO. ...
  • Bandara N, Tmiuk T. Isolation and identification of actinomycetes with ...
  • Setiawati S, Nuryastuti T, Sholikhah EN, Lisdiyanti P, Pratiwi SUT, ...
  • Brendecke JW, Axelson RD, Pepper IL. Soil microbial activity as ...
  • Ghosh S, Tripathi SK. Microbial succession and changes in carbon ...
  • Kumar V, Bisht GS, Gusain O. Terrestrial actinomycetes from diverse ...
  • Graça AP, Calisto R, Lage OM. Planctomycetes as novel source ...
  • Zhao LY, Shi J, Xu ZY, Sun JL, Yan ZY, ...
  • Eom JS, Lee SY, Choi HS. Bacillus subtilis HJ۱۸‐۴ from ...
  • Fei P, Xu Y, Zhao S, Gong S, Guo L. ...
  • Tian Y, Lu S, Zhou S, Li Z, Guan S, ...
  • Hanson BS, Hailemariam A, Yang Y, Mohamed F, Donati GL, ...
  • Kellogg JJ, Todd DA, Egan JM, Raja HA, Oberlies NH, ...
  • Quitério EG, Grosso C, Ferraz R, Delerue-Matos C, Soares C. ...
  • Bergmann S, Funk AN, Scherlach K, Schroeckh V, Shelest E, ...
  • Wasil Z, Pahirulzaman KA, Butts C, Simpson TJ, Lazarus CM, ...
  • Aisy ND, Wardani ARD, Paradhipta DHV, Agus A, Noviadi CT. ...
  • Wang J, Fan H, Han Y, Zhao J, Zhou Z. ...
  • Choi JJ, McCarthy MW. Cefiderocol: a novel siderophore cephalosporin. Expert ...
  • Rajchakit U, Sarojini V. Recent developments in antimicrobial-peptide-conjugated gold nanoparticles. ...
  • Waller AS, Clements JM. Novel approaches to antimicrobial therapy: peptide ...
  • Stach JE, Maldonado LA, Ward AC, Goodfellow M, Bull AT. ...
  • Ayuso-Sacido A, Genilloud O. New PCR primers for the screening ...
  • Metsä-Ketelä M, Salo V, Halo L, Hautala A, Hakala J, ...
  • Hajizadeh M, Pourahmad F, Nemati M. Isolation and screening of ...
  • نمایش کامل مراجع