Evaluation of antimicrobial effects of copper–zinc oxide nanocomposites biosynthesized by pome pomegranate peel on some pathogenic bacteria

Ever since humans started using antibiotics to treat bacterial diseases, resistance to treatment has been observed in bacteria. Studies have shown that by using metals we can take advantage of their antibacterial effects. The purpose of this research is to investigate the antibacterial activities of copper-zinc nanocomposite synthesized by green method (from pomegranate peel extract) on some pathogenic bacteria. Therfore, after green synthesis of copper-zinc oxide nanocomposite, the characteristics of nanocomposite were evaluated using UV-Vis spectrophotometry, X-ray diffraction analysis (XRD), FT-IR analysis, spectrum analysis (EDX) and scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Antibacterial activity of zinc-copper oxide nanocomposite against some pathogenic agents was investigated by well diffusion and blank disk methods in agar medium. The results of Well diffusion method were better compared to blank disc. It was demonstrated that the maximum inhibitory concentration on Staphylococcus aureus bacteria at concentrations of ۱۰۰, ۲۵۰, and ۵۰۰ µg/ml showed; diameters of the non-growth halo of ۱۷, ۱۶ and ۲۱ mm respectively. In contrast, the minimum inhibitory concentration on Enterococcus faecalis at concentrations of ۱۰۰, ۲۵۰, and ۵۰۰ µg/ml, showed the diameters of the non-growth halo of ۱۰, ۱۲, and ۱۴ mm respectively. The microbicidal properties of nanoparticles were investigated by two methods: the minimum growth inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). In the MIC method, the treatment concentration of ۰.۷۸ PPM stopped the growth of Escherichia coli and the treatment concentration of ۱.۵۶ PPM stopped the growth of other bacteria. MBC for Escherichia coli was ۱.۵۶ and for Pseudomonas aeruginosa, Shigella dysentery and Staphylococcus aureus was ۳.۱۲ PPM and had no effect on Enterococcus faecalis. These results showed that copper-zinc oxide nanocomposite synthesized from pomegranate peel inhibited the growth of the above-mentioned bacteria in all concentrations.Ever since humans started using antibiotics to treat bacterial diseases, resistance to treatment has been observed in bacteria. Studies have shown that by using metals we can take advantage of their antibacterial effects. The purpose of this research is to investigate the antibacterial activities of copper-zinc nanocomposite synthesized by green method (from pomegranate peel extract) on some pathogenic bacteria. Therfore, after green synthesis of copper-zinc oxide nanocomposite, the characteristics of nanocomposite were evaluated using UV-Vis spectrophotometry, X-ray diffraction analysis (XRD), FT-IR analysis, spectrum analysis (EDX) and scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Antibacterial activity of zinc-copper oxide nanocomposite against some pathogenic agents was investigated by well diffusion and blank disk methods in agar medium. The results of Well diffusion method were better compared to blank disc. It was demonstrated that the maximum inhibitory concentration on Staphylococcus aureus bacteria at concentrations of ۱۰۰, ۲۵۰, and ۵۰۰ µg/ml showed; diameters of the non-growth halo of ۱۷, ۱۶ and ۲۱ mm respectively. In contrast, the minimum inhibitory concentration on Enterococcus faecalis at concentrations of ۱۰۰, ۲۵۰, and ۵۰۰ µg/ml, showed the diameters of the non-growth halo of ۱۰, ۱۲, and ۱۴ mm respectively. The microbicidal properties of nanoparticles were investigated by two methods: the minimum growth inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). In the MIC method, the treatment concentration of ۰.۷۸ PPM stopped the growth of Escherichia coli and the treatment concentration of ۱.۵۶ PPM stopped the growth of other bacteria. MBC for Escherichia coli was ۱.۵۶ and for Pseudomonas aeruginosa, Shigella dysentery and Staphylococcus aureus was ۳.۱۲ PPM and had no effect on Enterococcus faecalis. These results showed that copper-zinc oxide nanocomposite synthesized from pomegranate peel inhibited the growth of the above-mentioned bacteria in all concentrations.

کلیدواژه ها:

pathogenic bacteria ، biosynthesis ، antibacterial activity ، copper and zinc oxide nanocomposite