Anticancer Activity of Silver Nanoparticles Combined with Beetroot Extract Synthesized via Non-Thermal Plasma Technology

This study presents new methods for the preparation of nanomaterials and extracts, where DC sputtering technology was adopted to obtain a solution of silver plasmon nanoparticles (AgNPs) and mix them with red beet extract prepared with cold-atmosphere plasma jet (CAPJ) technology. One of the important challenges in our research is the adoption of new, environmentally friendly, and fast technologies to support nanoparticles in the treatment of cancer and protect living cells from toxicity. AgNPs were prepared with different currents (۶ mA, ۸ mA, and ۱۰ mA) mixed with beetroot extract and applied to breast cancer cells, where their effectiveness in the diagnosis and treatment of cancer was evaluated. To determine the properties of silver, field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), RAMAN spectroscopy, and UV-Visible were studied. The effects of toxicity and its effectiveness in the diagnosis and treatment of cancer cells were also investigated. The best results were at a current of ۱۰ mA when applied to cancer cells. Furthermore, a comparison was made between the human breast cancer MDA-MB-۲۳۱ cell line treated with silver nanoparticles plus green materials (beetroot extract) as an anticancer agent (breast cancer) and the normal rat embryonic fibroblast (REF) cell line. Inhibition rates of ۵۳.۹۹% and ۸۰.۳۳%, respectively, were observed in the MDA breast cancer cell line, while cytotoxic effects on normal cells (REF) did not exceed ۱۸.۰۷% and ۱۷.۵%, respectively, indicating minimal side effects on healthy cells.

کلیدواژه ها:

AgNPs ، DC and CAPJ techniques ، Red beet extract ، breast cancer ، MDA cells line ، REF cells line ، growth inhibition