Evaluation Of L-Theanine`S Effect On Hepatic Enzyme Profiles In Cholestatic Rats

Cholestatic liver disease is a leading cause of global morbidity and mortality. The experimental model of bile duct ligation (BDL) is commonly applied to simulate cholestasis in laboratory studies. L-Theanine, classified as a non-protein amino acid, is predominantly synthesized within the roots of the green tea plant, Camellia sinensis, and subsequently concentrates within its foliage. This study aimed to evaluate L-Theanine`s effect on hepatic enzyme profiles in cholestatic rats. Forty adult male Wistar rats were randomly allocated into eight experimental groups of five animals each, comprising: ۱) Intact, ۲) BDL, ۳-۵) Intact + L-Theanine, ۶-۸) BDL + L-Theanine. Rats were orally administered L-Theanine solution (۱۰۰, ۲۰۰ or ۴۰۰ mg/kg body weight sequentially) via intra-gastric gavage (daily) for a ۳۰-day continuous period. At the end of this period, cardiac puncture was performed, followed by biochemical analysis. Our results demonstrated that BDL remarkably increased the plasma concentration of Alanine aminotransferase, Aspartate aminotransferase, Alkaline phosphatase, Gamma-glutamyl transferase, as well as total bilirubin levels. L-Theanine treatment in BDL rats markedly attenuated these biochemical changes. The greatest decrease in the concentration of these enzymes and total bilirubin was achieved with a dose of ۴۰۰ mg/kg body weight. These results indicate that L-Theanine can ameliorate cholestatic liver injury and hepatocellular damage caused by sequestered bile acids.

کلیدواژه ها:

Bile Duct Ligation ، Cholestasis ، L-Theanine ، Liver injury