First report of beet curly top Iran virus infecting cantaloupe in Iran

The family Geminiviridae comprises a large group of plant-infecting viruses with twined particles and one or two molecules of circular single-stranded DNA genome (Navas-Castillo & Fiallo-Olivé ۲۰۲۱). During a survey for identification of geminivirus-infecting crops in ۲۰۱۷, diseased cantaloupe plants (Cucumis melo L. subsp. melo) (n=۱۰) showing cup-shaped and crinkling leaves symptoms (Fig. ۱) were sampled from Sabzevar farms in the west of Khorasan Razavi Province, Iran. Total DNA were extracted from diseased samples using a CTAB method followed by enrichment of circular DNA molecules of the samples using rolling circle amplification (RCA) method and phi۲۹ DNA polymerase enzyme (TempliPhi, GE Healthcare, USA) as previously described by Shepherd et al. (۲۰۰۸). Subsequently, restriction pattern of the samples was analyzed using different enzymes. Due to identical restriction pattern of the resulted digested RCA product, the sample Ta-۱۰۹ was chosen and HindIII restricted RCA product (~۲.۹ kb in size) was cloned into the HindIII digested pGreen۰۰۲۹ plasmid followed by Sanger sequencing of a part (۹۷۶ bp) of the recombinant plasmid. The resulted sequence of the isolate Ta-۱۰۹ was used to search the database using basic local alignment search tool (BLASTn) (Altschul et al., ۱۹۹۰). Results indicated that beet curly top Iran virus (Becurtovirus betae, family Geminiviridae) is associated with the cantaloupe sample.

کلیدواژه ها:

Beet curly top Iran virus ، cantaloupe ، rolling circle amplification ، Sabzevar