Physicochemical Parameters Affecting the In-vitro Toxins Production by Characterized Antibiotic-resistant Clostridium Perfringens Toxinotype B Isolates

Background: Alpha, beta and epsilon toxins of Clostridium perfringens toxintype B are the major potent toxins involved in enterotoxemia. To combat this condition, proper vaccination of animals is required with an effective vaccine. Objectives: This research aimed to characterize the resistant C. perfringens type B isolates for in-vitro toxin production potential under the influence of various physicochemical parameters. Methods: C. perfringens isolates were characterized based on biochemical tests, toxinotyping, and ۱۶S rRNA typing. The indigenously characterized C. perfringens toxinotype B isolates (n=۶) were subjected to antibiotic susceptibility profiling through the Kirby-Bauer method. The resistant C. perfringens type B isolates were subjected to toxin production optimization under physicochemical parameters (physical: Temperature, pH and time of incubation; chemical: Glucose, vitamin-mineral mixture, tween ۸۰ and sodium salts at various concentration). Results: The C. perfringens isolates were identified as toxinotype B. Isolates MW۵۵۱۸۸۷.۱ and MW۳۳۲۲۴۷.۱ produced higher hemolytic and cytotoxic units of toxins at a ۰.۲% glucose concentration in the broth after ۲۴ hours at ۳۷ ºC, respectively. Alpha toxin, Beta toxin, Clostridium perfringens toxinotype B, Epsilon toxin, Glucose: To combat disease, controlling antibiotic resistance and ensuring proper vaccination of animals is crucial. These C. perfringens isolates may have commercial applications for toxoid vaccine production after further. Conclusion: To combat disease, control of antibiotic resistance and proper vaccination of animals is crucial. These C. perfringens isolates may have commercial application for toxoid vaccine production after further characterization and molecular testing of toxins.

کلیدواژه ها:

Alpha Toxin ، Beta toxin ، Clostridium perfringens toxinotype B ، Epsilon toxin ، glucose