Optimization of Edamame (𝐺𝑙𝑦𝑐𝑖𝑛𝑒 𝑚𝑎𝑥) Extract Essence Formula as a Natural Antioxidant and Anti-Tyrosinase Agent Using a Simplex Lattice Design

Edamame (Glycine max (L.) Merr.) is rich in bioactive compounds, particularly isoflavones, which offer beneficial effects on skin health as natural antioxidant and anti-tyrosinase agent. This study aimed to optimize an edamame extract-based essence using the Simplex Lattice Design (SLD) method, with Tween ۸۰ and xanthan gum as formulation variables. The extract demonstrated antioxidant and anti-tyrosinase activities with IC۵۰ values of ۲۰۲.۹۴۸ ± ۱.۵۲۴ µg/mL and ۴۲.۱۳۹ ± ۰.۶۷۶ µg/mL, respectively. The optimized formula contained ۹.۱۶۹% Tween ۸۰ and ۰.۸۳۱% xanthan gum. Predicted and observed values for key parameters (pH, viscosity, and spreadability) demonstrated no significant differences, indicating a reliable model fit. The final essence also exhibited good organoleptic and homogeneity characteristics. These resutls support the potential of edamame extract for formulation into effective cosmetic products with antioxidant and anti-tyrosinase properties.

کلیدواژه ها:

Edamame (Glycine max) ، Essence formulation ، Formulation optimization ، Antioxidant ، Antityrosinase